Autophagy Inhibitors Sensitized the Chemotherapy to PTX-resistant Ovarian Cancer Cells
Objective To explored the role of autophagy activation in paclitaxel(PTX)-resistant ovarian cancer cells and the effects of autophagy inhibition on the treatment of resistant cancer cells.Methods The ovarian cancer cell lines SKOV3 and PTX-resistant ovarian cancer cells were used.MTT assay was used to determine the cell viability.Apoptosis rates were detected by Annexin V/PI staining assay.The protein expression was determined by western blot(WB).The cells were transfected by LC3-GFP-RFP using lipofectamine 2000 and the fluorescence was detected by laser scanning confocal microscope.Results The inhib-itory effect of PTX on SKOV3/PTX cells was significantly reduced compared with SKOV3 cells and inhibitory concentration50%(IC50)of PTX on SKOV3 cells and SKOV3/PTX cells were 41.91±2.92 μM and 114.6±17.6 μM,respectively.The apoptot-ic effect of PTX on SKOV3/PTX cells was significantly reduced.It was proved that SKOV3/PTX cells showed increased autophagy activation after LC3-GFP-RFP overexpression through detecting the expression of LC3 and P62.Autophagy of SKOV3/PTX cells was inhibited after treatment with autophagy inhibitors,chloroquine(CQ)and 3-MA and the inhibitory effect and apoptosis-indu-cing ability of PTX on SKOV3/PTX cells was improved.Conclusion Autophagy activation has a protective role in the PTX-re-sistant ovarian cancer cells.Inhibition of autophagy enhanced the cytotoxic effect of PTX-resistant ovarian cancer cells.
Ovarian cancerPTX-resistant cancer cellsPTXAutophagySKOV3
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维普
