Analysis of Biological Behaviors of Human Breast Cancer Cell Line Under the Regulation of β-ionone Via Caspase-3 Signaling Pathway
Objective To investigate the regulation of β-ionone on regulating the biological behavior of breast cancer cells through caspase-3 signaling.Methods Human breast cancer MCF-7 cells were divided into blank control group and the ex-perimental group.The experimental group was further divided into 4 subgroups according to the different concentrations of β-io-none,25 μmol/L,50 μmol/L,100 μmol/L and 200 μmol/L subgroups.CCK-8 and trypan blue exclusion methods were used to detect the effects of β-ionone on the proliferation and growth curve of MCF-7 cells.Hoechst 33258 fluorescent staining was used to observe the effects of β-ionone on the apoptotic morphology of MCF-7 cells.Meantime,RT-PCR and Western Blot were used to detect the effect of β-ionone on the mRNA and protein expressions of Caspase-3.Results Compared with blank control group,the optical density(OD)values of breast cancer MCF-7 cells treated with different concentrations of β-ionone was significantly decreased(P<0.05).The proliferation inhibition rates of MCF-7 cells were 7.92%,28.96%,45.22%and 56.83%under the treatment with β-ionone at the concentrations of 25 μmol/L,50 μmol/L,100 μmol/L and 200 μmol/L in experimental groups,re-spectively.The cell counts of MCF-7 cells treated with different concentrations of β-ionone was significantly lower than that of blank control group from the second day of treatment(P<0.05).During the 7 days of treatment,MCF-7 cell counts in blank con-trol group and 25 μmol/L subgroup showed an increasing trend over time,while MCF-7 cell counts in 50 μmol/L,100 μmol/L and 200 μmol/L subgroups showed a decreasing trend.Hoechst 33258 fluorescence staining showed that human breast cancer MCF-7 cells treated with β-ionone showed varying degrees of apoptosis compared to blank control group,with the most obvious ap-optosis occurring in cells treated with β-ionone at a concentration of 200 μmol/L.The mRNA and protein expression levels of Caspase-3 in MCF-7 cells treated with different concentrations of β-ionone increased significantly compared to blank control group(P<0.05),and the increase was in a concentration-dependent manner(P<0.05).Conclusion β-ionone may inhibit the pro-liferation and growth of breast cancer cells and promote their apoptosis by activating the Caspase-3 signaling pathway,thereby ex-erting a tumor suppressor effect.
β-iononeHuman breast cancer MCF-7 cellsCell proliferationGrowth curveCell apoptosisCaspase-3 sig-naling pathway
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