Expression and Clinical Significance of TRAF4 and Eg5 in Ovarian Cancer
Objective To investigate the expression and clinical significance of TRAF4 and Eg5 in ovarian cancer.Methods Paraffin-embedded tissue specimens were collected from 65 patients with ovarian cancer undergoing surgical treat-ment.36 patients with benign ovarian epithelial lesions undergoing surgical treatment and 36 normal ovarian tissues undergoing to-tal hysterectomy and adrenal resection due to uterine fibroids were selected.Immunohistochemical method was used to detect the positive expression of TRAF4 and Eg5 in tissues.Real time fluorescent quantitative PCR was used to detect the expression of TRAF4 and Eg5 mRNA.Person correlation was used to analyze the correlation between TRAF4 and Eg5 levels.The diagnostic ef-ficacy of TRAF4 and Eg5 levels in ovarian cancer was analyzed by drawing the receiver operating characteristic curve(ROC).Ka-plan Meier curve was used to analyze the survival of ovarian cancer patients.Results The levels of TRAF4 and Eg5 mRNA in patients with lymph node metastasis were significantly higher than those without lymph node metastasis(P＜0.001);The levels of TRAF4 and Eg5 mRNA in tissues with lymph node metastasis were significantly higher than those in tissues without lymph node metastasis(P＜0.001).The levels of TRAF4 and Eg5 mRNA in FIGO stageⅢ～Ⅳwere significantly higher than those in stageⅢ～Ⅳ(P＜0.001).The levels of TRAF4 and Eg5 mRNA in T3-T4 stage were significantly higher than those in T1-T2 stage(P＜0.001);The levels of TRAF4 and Eg5 were positively correlated(γ =0.326,P＜0.001);The ROC curve area of TRAF4 was 0.837(95%CI:0.765-0.908,P＜0.001).When the cut-off value was 1.36,the sensitivity was 73.85%,and the specificity was 81.54%;The area of the ROC curve of Eg5 was0.913(95%CI:0.864-0.961,P＜0.001).When the cut-off value was1.48,the sensitivity was 87.69%,and the specificity was 81.54%;The 5-year overall survival rate of patients with high TRAF4 expres-sion was47.06%,which was significantly lower than that of patients with low TRAF4 expression 67.74%,(P＜0.05);The 5-year overall survival rate of high TRAF4 expression group was 47.06%,significantly lower than that of low TRAF4 expression group(67.74%)(P＜0.05).The5-year overall survival rate of high Eg5 expressiongroupwas42.86%,whichwas significantly lower than that of low Eg5 expression group(73.33%)(P＜0.05).Conclusion TRAF4 and Eg5 are highly expressed in ovari-an cancer,which is related to lymph node metastasis,FIGO stage and invasion degree.Detection of TRAF4 and Eg5 expression has certain diagnostic value for early diagnosis and prognosis of ovarian cancer.
万方数据
维普
