Expression and Correlation of RNA XIST and mir-130b-3p in Breast Cancer Cells
Objective To investigate the expression of RNA XIST and mir-130b-3p in breast cancer and their correlation with clinicopathologic features and prognosis.Methods The levels of RNA XIST and mir-130b-3p were measured by immunohis-tochemical staining in 120 patients with breast cancer.All patients were followed up for 60 months from the beginning of treat-ment,and the overall survival rate was recorded.Logistic multiple regression equation was established to analyze the high-risk fac-tors of death in patients with breast cancer.Results The expression of RNA XIST was higher in breast cancer tissues than in ad-jacent normal tissues(P＜0.05),and the expression of mir-130b-3p was lower in breast cancer tissues than in adjacent normal tissues(P＜0.05).The expression of RNA XIST and mir-130b-3p was significantly negatively correlated in breast cancer tissues(P＜0.05).The expression of RNA XIST and mir-130b-3p was significantly correlated with age,tumor diameter,tumor stage,lymph node metastasis and differentiation(P＜0.05).All patients were followed up for 60 months,6 cases had recurrence 24-36 months after treatment,8 cases had systemic metastasis,54 cases died,RNA XIST expression in survival patients was lower than death patients(P＜0.05),mir-130b-3p expression in survival patients was higher than death patients(P＜0.05).Age≥55 years,tumor diameter＞5 cm,tumor stage Ⅲ～Ⅳ,lymph node metastasis,high RNA XIST expression,and low mir-130b-3p expression were risk factors for death in breast cancer patients(P＜0.05).Conclusion RNA XIST and mir-130b-3p are signifi-cantly correlated with the prognosis of breast cancer patients,and their expression levels in cancer cells are negatively correlated.They can be monitored as biomarkers and therapeutic targets for early screening of breast cancer.
万方数据
维普
