The Expressional Circadian Rhythms of Clock Genes Bmal1 and Per2 in Raji、Hut-78、OCI-LY8 and HL-60 Cell Lines
Objective To detect the circadian rhythms of the expressional levels of Bmal1 and Per2 genes in hematologi-cal tumor cell lines Raji、Hut-78、OCI-LY8 and HL-60,and to explore the possible connections between them and the occurrence and development of hematological tumor.Methods The transcriptional levels of Bmal1 and Per2 at different times of the day in Raji、Hut-78、OCI-LY8 and HL-60 cell lines were measured by real-time PCR after the induction of synchronous rhythms of the clock gene expression.Cosinor analysis was performed by fitting transcription levels of genes at individual time points to predict the peak and trough periods of gene transcription in different cell lines.Results Circadian rhythms in the transcriptional level of Bmal1 and Per2 were observed in OCI-LY8、Hut-78、and HL-60 cell lines(P<0.05);Nocircadian rhythm was found in the tran-scriptional level of Bmal1 in Raji cell lines while the circadian rhythm of Per2 in it was found(P<0.05).Conclusion Circadi-an rhythms of the transcriptional levels of Bmal1 and/or Per2 could be found in various kinds of hematological tumor cell lines,in-dicating that it may take part in the occurrence and development of hematological tumors.
万方数据
维普
