摘要
目的 分析血管内皮细胞生长因子受体2(VEGFR2)、微小RNA-21(miR-21)、解偶联蛋白1(UCP1)、解偶联蛋白3(UCP3)在非小细胞肺癌(NSCLC)组织内的表达及与其预后的相关性.方法 选取98例NSCLC患者,术中取其癌组织与癌旁正常组织,检测VEGFR2、miR-21、UCP1及UCP3表达;分析VEGFR2、miR-21、UCP1及UCP3表达与其临床病理特征的关系;随访1年,分析VEGFR2、miR-21、UCP1及UCP3表达与患者生存率的关系.结果 癌组织内的VEGFR2、UCP1阳性表达率及miR-21相对表达量高于癌旁正常组织,UCP3阳性表达率低于癌旁正常组织,差异有统计学意义(P＜0.05);VEGFR2、miR-21、UCP1及UCP3表达与淋巴结转移、临床分期、分化程度有关(P＜0.05);VEGFR2、UCP1阳性表达及miR-21高表达患者的1年生存率分别低于VEGFR2、UCP1阴性表达及miR-21低表达患者,UCP3阳性表达患者的1年生存率高于UCP3阴性表达者,差异有统计学意义(P＜0.05).结论 VEGFR2、miR-21、UCP1及UCP3在NSCLC癌组织内呈异常表达,与患者的预后具有紧密联系.
Abstract
Objective To analyze the expression of vascular endothelial growth factor receptor 2(VEGFR2),microRNA-21(miR-21),uncoupling protein 1(UCP1),and uncoupling protein 3(UCP3)in non-small cell lung cancer(NSCLC)tissues and their correlation with prognosis.Methods 98 NSCLC patients were selected,and their cancer tissue and adjacent normal tissue were taken during surgery to detect the expression of VEGFR2,miR-21,UCP1,and UCP3;Analyze the relationship between the expression of VEGFR2,miR-21,UCP1,and UCP3 and their clinical pathological characteristics;Follow up for 1 year to analyze the relationship between the expression of VEGFR2,miR-21,UCP1,and UCP3 and patient survival rate.Results The positive expression rates of VEGFR2 and UCP1,as well as the relative expression levels of miR-21 in cancer tissue were higher than those in normal tissue adjacent to cancer,while the positive expression rate of UCP3 was lower than that in normal tissue adjacent to cancer,with statistical significance(P＜0.05);The expression of VEGFR2,miR-21,UCP1,and UCP3 is related to lymph node metastasis,clinical stage,and differentiation degree(P＜0.05);The 1-year survival rate of patients with positive expression of VEGFR2,UCP1,and high expression of miR-21 was lower than that of patients with negative expression of VEGFR2,UCP1,and low expression of miR-21,respectively.The 1-year survival rate of patients with positive expression of UCP3 was higher than that of patients with negative expression of UCP3,and the difference was statistically significant(P＜0.05).Conclusion VEGFR2,miR-21,UCP1,and UCP3 are abnormally expressed in NSCLC cancer tissue,which is closely related to the prognosis of patients.
维普
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