Objective To study the effect of Astragalus polysaccharide(APS)on the proliferation of Siha cells with cer-vical cancer and the expression of phosphorylated histone H2AX(γH2AX)after radiotherapy.Methods Siha cells were cul-tured in vitro,and the 2 groups were divided into radiotherapy group and Astragalus polysaccharide combined with radiotherapy group.Cell proliferation was analyzed by CCK 8,and γH2AX expression level after radiotherapy was measured by Western blot-ting.Results The higher concentration of APS led to weaker proliferation of Siha cells;With longer period exposed of APS,the less proliferation compared with the radiotherapy group;The expression level of γH2AX in the APS combined with radiotherapy group suggested was higher than that in the radiotherapy group at 4 hours after radiotherapy.Conclusion APS can inhibit the proliferation of Siha cells,and promote the expression of γH2AX,maybe can improve the sensitivity of radiotherapy.
关键词
黄芪多糖/Siha细胞/DNA损伤/γH2AX/放疗敏感性
Key words
Astragalus polysaccharide/Siha cells/DNA damage/Histone H2AX/Sensitivity of radiotherapy
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