The Effect of miR-150-5p Targeting ZEB1 to Regulate EMT on the Malignant Biological Behavior of Endometrial Carcinoma Cells
Objective To investigate whether microRNA-150-5p(miR-150-5p)can target zinc finger E-box binding pro-tein 1(ZEB1)to regulate epithelial mesenchymal transformation(EMT)of endometrial carcinoma(EC)cells,thereby affecting the malignant biological behavior of cancer cells.Methods The relative expression of miR-150-5p in normal endometrium and EC tissues,endometrial epithelial cell lines hEEC,EC cell lines Ishikawa and HEC-1-A were detected by RT-qPCR.Overexpres-sion of miR-150-5p,MTT assay,colony formation test,wound healing test and Transwell test were used to evaluate the viability,clonal formation,migration and invasion of Ishikawa cells.Double luciferase reporter gene experiment verified the targeting rela-tionship between miR-150-5p and ZEB1.The relative expression of miR-150-5p and ZEB1 mRNA was detected by RT-qPCR.The expressions of ZEB1,E-cadherin,N-cadherin,vimentin and matrix metalloproteinase-9(MMP-9)were detected by Western blot.Results Compared with normal endometrial tissue,the relative expression of miR-150-5p in EC tissue was decreased(P＜0.05).Compared with hEEC cells,the relative expression of miR-150-5p in HEC-1-A cells and Ishikawa cells decreased(P＜0.05),and that in Ishikawa cells was the lowest(P＜0.05).Compared with the blank group,the absorbance value at 490 nm,the number of colony,migration and invasion,the relative expression of ZEB1 mRNA and protein,and the relative expression of N-cadherin,Vimentin and MMP-9 protein in miR-150-5p mimics group significantly decreased,while the relative expression of miR-150-5p and E-cadherin protein significantly increased(P＜0.05).According to bioinformatics analysis,ZEB1 was predicted as a potential target gene of miR-150-5p.Conclusion miR-150-5p can target ZEB1 to inhibit the malignant biological behavior of cancer cells,and its mechanism may be related to the regulation of EMT progression of EC cells.
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