Study on the LncRNA TUG1 Facilitating Ovarian Cancer Cell Proliferation and Metastasis via Targeting the AKT/mTOR Signaling Pathway
Objective To explore the effect and the possible mechanism of long noncoding RNA taurine-upregulated gene 1(lncRNA TUG1)on the proliferation and metastasis of ovarian cancer cells by mediating protein kinase B/mammalian tar-get of rapamycin(AKT/mTOR)signaling pathway.Methods The pairs of ovarian cancer tissues and adjacent normal tissues were collected from 112 ovarian cancer patients.Reverse transcription-polymerase chain reaction(RT-PCR)was used to detect the expression of TUG1 in the selected tissues,ovarian cancer cell lines(OC3,SKOV3,A2780,HO-8910)and human ovarian normal epithelial cell line IOSE80.SKOV3 cells were assigned to either TUG1 shRNA transfection(si-TUG1 group)or empty vector plas-mid transfection(NC group),thereafter,the ovarian cancer cell proliferation,cycle and metastasis profiles were detected using CCK8 assay,flow cytometry and scratch test.The relative expression levels of AKT,mTOR,phosphorylated AKT(p-AKT),phos-phorylated mTOR(p-mTOR),cell cycle-related proteins(Cyclin D1,Cyclin B1,CDK6),and metastasis-related proteins(MMP-2,Snail)in each group were measured by Western blot.Results RT-PCR denoted that TUG1 expression was higher in ovarian cancer tissues than in adjacent normal tissues(P＜0.05).TUG1 expression was higher in ovarian cancer cell lines OC3,SKOV3,A2780,and HO-8910 than in IOSE80 cells,and TUG1 expression was the highest in SKOV3(P＜0.05).The cell proliferation,cell cycle arrest at G2/M phase and cell migration distance were all smaller in si-TUG1 group than in NC group,and the cell cycle arrest at G0/G1 phase was higher in si-TUG1 group than in NC group(P＜0.05).The protein expression of p-AKT/AKT,p-mTOR/mTOR,Cyclin D1,Cyclin B1,CDK6,MMP-2,and Snail in si-TUG1 group was lower than those in NC group(P＜0.05).Conclusion Down-regulation of lncRNA TUG1 expression can attenuate the proliferation and metastasis ability of ovarian cancer cells,which may be related to the inhibition of the AKT/mTOR signaling pathway.
Long noncoding RNA taurine-upregulated gene 1Ovarian cancerCell proliferationAKT/mTOR
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