Effect of lncRNA CCAT1 via miR-490-3p on Chemoresistance of Bladder Cancer Cells
Objective To explore the impact of the long non-coding RNA(lncRNA)CCAT1 through miR-490-3p on chemotherapy resistance in bladder cancer cells.Methods A cisplatin-resistant cell line derived from bladder cancer cells(T24)was established.A luciferase reporter assay was conducted to investigate the interaction between lncRNA CCAT1 and miR-490-3p,as well as miR-490-3p and KDM7A mRNA-UTR.Subsequently,the apoptosis levels in cisplatin-resistant cell lines were as-sessed following the knockdown of lncRNA CCAT1,KDM7A,or overexpression of miR-490-3p.Results The apoptosis level in the cisplatin-resistant bladder cancer cell line T24 exhibited a significant decrease after cisplatin treatment compared to the control bladder cancer cell line T24(P＜0.05).Following cisplatin treatment,the knockdown of LncRNA CCAT1 led to a notable in-crease in the apoptosis level in the cisplatin-resistant bladder cancer cell line T24(P＜0.05).Upon overexpression of miR-490-3p,a luciferase reporter assay demonstrated a decrease in luciferase activity in LncRNA CCAT1 wild type compared to LncRNA CCAT1 mutant(P＜0.05).Furthermore,overexpression of miR-490-3p resulted in a significant increase in the apoptosis level of the cisplatin-resistant bladder cancer cell line T24 after cisplatin treatment(P＜0.05).In addition,a luciferase reporter assay re-vealed reduced luciferase activity in KDM7A-3 UTR wild type compared to KDM7A-3 UTR mutant after overexpression of miR-490-3p(P＜0.05).The mRNA and protein expression levels of KDM7A in the cisplatin-resistant bladder cancer cell line T24 de-creased significantly after overexpression of miR-490-3p(P＜0.05),while they increased after knockdown of miR-490-3p(P＜0.05).Subsequent to KDM7A knockdown,cisplatin treatment significantly increased the apoptosis level in the cisplatin-resistant bladder cancer cell line T24(P＜0.05).Conclusion The lncRNA CCAT1/miR-490-3p/KDM7A axis facilitated the resistance of bladder cancer cells to chemotherapeutic drugs.
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