Changes and Clinical Significance of PD-1/PD-L1 and Related Immune Cells in Peripheral Blood and Tumor Tissues of Patients with Colorectal Cancer
Objective To investigate the changes and clinical significance of programmed cell death protein 1(PD-1)/programmed cell death-ligand 1(PD-L1)and related immune cells in peripheral blood and tumor tissues of patients with colorec-tal cancer.Methods 80 patients underwent radical resection of colorectal cancer were included in cancer group,and another 80 healthy patients underwent physical examination during the same period were enrolled as the control group.Peripheral venous blood was collected from both groups.The expression levels of PD-1,PD-L1,CD4+CD25+regulatory T cells(CD4+CD25+Tregs)and fork head/winged helix transcription factor p3(Foxp3)were measured.The cancer tissues and adjacent normal tissues were collected during surgery,and the expression levels of PD-1,PD-L1 and Foxp3 proteins in the tumor tissues and adjacent tis-sues were detected by immunohistochemistry.Results The expression levels of PD-1,PD-L1 and Foxp3 in peripheral blood of cancer group were higher than those of the control group(P＜0.05).After surgery,the expression levels of PD-1,PD-L1 and Foxp3 in peripheral blood were decreased in cancer group(P＜0.05).The positive expression rates of PD-1,PD-L1 and Foxp3 in cancer tissues were higher than those in adjacent normal tissues(P＜0.05).The positive expression of PD-1 in cancer tissues was correlated with the depth of tumor invasion,lymph node metastasis and pTNM stage(P＜0.05).The positive expression of PD-L1 in cancer tissues was correlated with tumor location and depth of invasion(P＜0.05).The positive expression of Foxp3 Treg was correlated with tumor differentiation,depth of invasion and lymph node metastasis(P＜0.05).Conclusion PD-1,PD-L1 and Treg cells are all associated with the immune microenvironment of colorectal cancer and have been linked to the occurrence and development of colorectal cancer,and the combined detection of the three indicators can be used to evaluate the response to im-mune checkpoint inhibitors in colorectal cancer patients.
Colorectal cancerPD-1PD-L1Regulatory T cellsFoxp3Pre-and post-operationClinical significance
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