山奈酚对高糖诱导的人肾上皮细胞增殖与凋亡的影响
Effects of Kaempferol on Proliferation and Apoptosis of Human Renal Epithelial Cells Under High Glucose
喻信林 1宋莹 1张宇晴 1颜伟 1周黄燕 1邹学森1
作者信息
摘要
目的 探究山奈酚对高糖诱导人肾上皮细胞(Hkb20)增殖及凋亡的影响.方法 分组培养人肾上皮细胞分为正常糖浓度组(对照组)、高糖组、氯沙坦组(阳性对照)和不同浓度山奈酚组(10 μmol/L、30 μmol/L、50 μmol/L山奈酚与高糖共同作用).采用CCK-8法和AV/PI法应用流式细胞仪分别检测细胞增殖和凋亡情况,qRT-PCR检测TGF-β1和Smad3 mRNA水平的表达,Western blot法检测细胞因子TGF-β1和Smad3蛋白含量.结果 高糖能诱导人肾上皮细胞增殖降低,使细胞凋亡率升高,增加TGF-β1和Smad3 mRNA和蛋白的表达水平(P＜0.05).不同浓度山奈酚处理后,山奈酚能显著增强细胞增殖和降低细胞凋亡率.同时TGF-β1和Smad3 mRNA和蛋白的表达水平下降,且呈剂量依赖性(P＜0.05).结论 山奈酚可逆转高糖诱导的人肾上皮细胞增殖降低和凋亡增加.这说明山奈酚可能是通过TGF-β1/Smad3信号通路抑制高糖环境下人肾上皮细胞的增殖降低和凋亡增加,从而最终导致肾脏纤维化.
Abstract
Objective To investigate the effects of kaempferol on the proliferation and apoptosis of human renal epitheli-al cells(Hkb20)induced by high glucose,and to clarify its related mechanisms.Methods Human renal epithelial cells were cultured in groups,including normal glucose group(control group),high glucose group,losartan group(positive control)and dif-ferent concentrations of kaempferol groups(10μmol/L,30μmol/L,50μmol/L kaempferol and high glucose co-action).The cell proliferation and apoptosis were detected by CCK-8 method and AV/PI method flow cytometry,the expression of TGF-β1 and Smad3 mRNA was detected by qRT-PCR,and the protein content of TGF-β1 and Smad3 was detected by Western blot.Results High glucose could induce the decrease of human renal epithelial cell proliferation,increase the apoptosis rate,and increase the expression levels of TGF-β1 and Smad3 mRNA and protein(P＜0.01).After different concentrations of kaempferol treatment,kaempferol could significantly increase cell proliferation and decrease apoptosis rate.At the same time,the expression levels of TGF-β1 and Smad3 mRNA and protein decreased,and showed a dose-dependent manner(P＜0.05).Conclusion Kaempferol could reverse the decrease of human renal epithelial cell proliferation and increase of apoptosis induced by high glucose.This sug-gests that kaempferol may inhibit the decrease of human renal epithelial cell proliferation and increase of apoptosis under high glu-cose environment through TGF-β1/Smad3 signaling pathway,which can eventually lead to renal fibrosis.
关键词
山奈酚/高糖/人肾上皮细胞/TGF-β1/Smad3/纤维化/糖尿病肾病Key words
Kaempferol/High glucose/Human renal epithelial cells/TGF-β1/Smad3/Fibrosis/Diabetic nephropathy引用本文复制引用
基金项目
江西省中医药管理局科技计划项目(2019A158)
江西省卫生健康委项目(202310928)
江西省肿瘤医院院内科研开放基金项目(KFJJ2023YB03)
出版年
2024
NETL
NSTL
万方数据