Objective To investigate the effects of kaempferol on the proliferation and apoptosis of human renal epitheli-al cells(Hkb20)induced by high glucose,and to clarify its related mechanisms.Methods Human renal epithelial cells were cultured in groups,including normal glucose group(control group),high glucose group,losartan group(positive control)and dif-ferent concentrations of kaempferol groups(10μmol/L,30μmol/L,50μmol/L kaempferol and high glucose co-action).The cell proliferation and apoptosis were detected by CCK-8 method and AV/PI method flow cytometry,the expression of TGF-β1 and Smad3 mRNA was detected by qRT-PCR,and the protein content of TGF-β1 and Smad3 was detected by Western blot.Results High glucose could induce the decrease of human renal epithelial cell proliferation,increase the apoptosis rate,and increase the expression levels of TGF-β1 and Smad3 mRNA and protein(P＜0.01).After different concentrations of kaempferol treatment,kaempferol could significantly increase cell proliferation and decrease apoptosis rate.At the same time,the expression levels of TGF-β1 and Smad3 mRNA and protein decreased,and showed a dose-dependent manner(P＜0.05).Conclusion Kaempferol could reverse the decrease of human renal epithelial cell proliferation and increase of apoptosis induced by high glucose.This sug-gests that kaempferol may inhibit the decrease of human renal epithelial cell proliferation and increase of apoptosis under high glu-cose environment through TGF-β1/Smad3 signaling pathway,which can eventually lead to renal fibrosis.
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