Expression and Correlation of miR-26,TGF-β_1 and REG γ in Endometrial Cancer Patients
Objective To investigate the expression and correlation of microRNA-26(miR-26),Transforming growth fac-tor β1(TGF-β1)and proteasome activator 11S regulator complex gamma subunit(REG γ)in endometrial cancer.Methods 60 cases of endometrial carcinoma(endometrial carcinoma group),20 cases of endometrial hyperplasia(endometrial hyperplasia group)and 20 cases of normal proliferative endometrium(normal group)were collected.Real-time quantitative PCR(RT-qPCR)was used to detect the expression of miR-26,TGF-β1 and REG γin endometrial tissue.The expression levels of miR-26,TGFB1 and REGγ were compared among the 3 groups,and the clinicopathologic features of the endometrial cancer were collected to ana-lyze the relationship between the clinicopathological parameters and the expression levels of miR-26,TGFB1 and REG γ.The ex-pression levels of miR-26,TGF-β1 and REG γ in endometrial cancer tissues were analyzed by Pearson's method The diagnostic value of miR-26,TGF-β1 and REG γ in endometrial cancer was analyzed by receiver operating characteristics curve(ROC).Re-sults The expression levels of miR-26 in normal group,endometrial hyperplasia group and endometrial carcinoma group de-creased in turn,while the expression levels of TGF-β1 and REG γ increased in turn,there was significant difference among the 3groups(P＜0.001).The expression of miR-26,TGF-β1 and REG γ in the pathological tissues of endometrial cancer patients was correlated with clinical stage,histological differentiation and lymph node metastasis(P＜0.05),but not with the degree of myometrial invasion(P＞0.05).The expression of miR-26 was negatively correlated with the expression of TGF-β1 and REG γ in endometrial cancer patients(P＜0.001),while the expression of TGF-β1 and REG γ was positively correlated(P＜0.001).The expression level of miR-26 was negatively correlated with clinical stage and positively correlated with differentiation(P＜0.001),and the expression level of TGFB1 and REGγ was positively correlated with clinical stage and negatively correlated with differenti-ation(P＜0.001).The AUC of miR-26,TGF-β1 and REGγ were 0.705,0.7450,0.760,respectively,which was lower than 0.861 of the combined diagnostic endometrial cancer in endometrial cancer patients.Conclusion The abnormal expression of miR-26,TGF-β1 and REGγ in endometrial carcinoma tissue is related to clinical stage and degree of tissue differentiation,and they are interrelated,so the combination of them has significant clinical value in diagnosing endometrial carcinoma.
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