Changes of Peripheral Blood DC Cells and Serum sIL-2R and HMGB-1 Levels in Pa-tients with Lung Cancer Before and After Radiotherapy and Their Clinical Significance
Objective To investigate the changes and clinical significance of peripheral blood DC cells and serum sIL-2R and HGMB-1 levels in patients with lung cancer before and after radiotherapy.Methods A total of 124 patients with lung cancer were collected and treated with intensity modulated radiation therapy(IMRT).4 ml of fasting venous blood was extracted before and after radiotherapy,and the levels of peripheral blood DC cells(DC 1,DC2)were detected by immunomagnetic beads method.The levels of serum sIL-2R and HMGB-1 were detected by double antibody sandwich enzyme-linked immunoassay.Pe-ripheral blood DC cells and serum sIL-2R and HMGB-1 levels in patients with lung cancer were counted according to gender,age,smoking status,pathological type,operation status,KPS score and clinical stage,and the relationship between patients'prognosis and serum sIL-2R and HMGB-1 levels was analyzed.Univariate and multivariate COX regression analysis was performed to ana-lyze the prognostic factors of patients with lung cancer.Results The level of peripheral blood DC cells in patients with lung cancer before and after radiotherapy was compared,P＞0.05.The levels of serum sIL-2R and HMGB-1 in patients with lung canc-er after treatment were compared with those before treatment,P＜0.05.Before treatment,the level of serum sIL-2R was compared in different gender,age,smoking or not,pathological type(P＞0.05),and in operation or not,different KPS score,clinical stage and lymph node metastasis(P＜0.05).Serum HMGB-1 levels were compared in different pathological types(P＞0.05),and in different gender,age,smoking,surgery,KPS score,clinical stage and lymph node metastasis(P＜0.05).After treatment,the level of serum sIL-2R was compared in different gender,age,smoking or not,pathological type(P＞0.05),and in operation or not,dif-ferent KPS score,clinical stage,lymph node metastasis(P＜0.05).Serum HMGB-1 levels were compared in different gender,age,smoking,pathological type,surgery,KPS score,clinical stage,lymph node metastasis,P＜0.05.The levels of serum sIL-2R and HMGB-1 in death group were higher than those in survival group(P＜0.05).DC2 in death group was lower than that in sur-vival group(P＜0.05).Univariate and multivariate COX regression analysis showed that clinical stage(Ⅲ～Ⅳ stage),lymph node metastasis,abnormal increase of sIL-2R and HMGB-1 level,and decrease of DC2 ratio were independent risk factors for poor prognosis of lung cancer patients.Conclusion There is no significant change in peripheral blood DC cells before and after radio-therapy in patients with lung cancer,but the levels of serum sIL-2R and HMGB-1 are significantly lower after treatment than be-fore treatment.The abnormal increase in the levels of sIL-2R and HMGB-1 and the decrease in the proportion of DC2 are inde-pendent risk factors for the prognosis of patients,and can predict the prognosis.
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