Effects of TNF-α on the Biological Behavior of Lung Cancer Cells by Regulating NF-κB/PXR Inflammatory Pathway and Its Mechanism
Objective To analyze TNF-α Regulating NF-κ The effect of B/PXR inflammatory pathway on the biological behavior of lung cancer cells and its mechanism.Methods A549 cells were divided into the control group and the intervention group.The control group was cultured without any intervention,and the intervention group was treated with human TNF-α Co cul-ture with 10 ng/ml for 12 hours;Cell proliferation,cell cycle,apoptosis and invasion were detected;PCR and WB methods were used to detect NF in the cells-K B.PXR protein and mRNA expression levels.Results At 24 h,48 h,and 72 h after TNF-α in-tervention,the absorbance values of cells in the intervention group were significantly higher than those in the control group,and the difference was statistically significant(P＜0.05);after 24 h of TNF-α intervention,the apoptosis rate of A549 cells in the in-tervention group was significantly lower than that in the control group,and the difference was statistically significant(P＜0.05);after 24 h of intervention,the S-phase proportion of cells treated with TNF-α in the intervention group was significantly higher than that in the control group,while the proportion of G2+M and Go+G,phase cells was significantly lower than that in the control group,and the difference was statistically significant(P＜0.05);after 24 h of intervention,the number of invasive cells treated with TNF-α in the intervention group was significantly higher than that in the control group,and the difference was statistically significant(P＜0.05);after 24 h of intervention,the expression levels of NF-κB and PXR proteins in cells treated with TNF-α in the intervention group were significantly higher than those in the control group,and the difference was statistically significant(P＜0.05);after 24 h of intervention,the expression levels of NF-κB and PXR mRNA in cells treated with TNF-α in the intervention group were significantly higher than those in the control group,and the difference was statistically significant(P＜0.05).Conclu-sion TNF-α can regulate the proliferation,apoptosis,and invasion of lung cancer cells by regulating the NF-κB/PXR inflamma-tory pathway.
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