The Role and Regulatory Mechanism of Kallikrein 10 in Regulating the Progression of Papillary Thyroid Carcinoma
Objective To explore the role and underline mechanism of kallikrein-related peptidase 10(KLK10)in pa-pillary thyroid carcinoma(PTC).Methods RT-qPCR and western blot assays were used to detect the expression of KLK10 in PTC tissues and cells.The cell viability and apoptosis rate were detected by CCK-8 and Annexin-V/PI apoptosis detection kits.Besides,glucose uptake,lactate production and ATP concentration were measured in different groups.The FAK/SRC/PI3K/AKT axis related proteins were examined using western blot assay.Results The mRNA and protein expression of KLK10 were in-creased in PTC tissues and cells,compared with normal tissues and cells(P＜0.01).Cell viability in KLK10-silenced group was weaker than control group and cell apoptosis rate was stronger than control group(P＜0.01).Meanwhile,compared with the si-NC group,the glucose uptake,lactate production and ATP concentration were markedly decreased in si-KLK10 group(P＜0.01).The results in the KLK10 overexpression group were opposite to those in the KLK10-silenced group.Moreover,compared with the con-trol group,the phosphorylation levels of FAK,SRC,PI3K and AKT were increased in KLK10 overexpression group.Conclusion KLK10 regulated the glycolysis and progression of papillary thyroid carcinoma patients via FAK/SRC/PI3K/AKT axis.
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