Mechanism of miR-15a-5p/CCND1 Pathway in Metastatic Invasion of Pancreatic Cancer Cells
Objective To explore the regulatory role of miR-15a-5p/CCND1 pathway in pancreatic cancer.Methods CAPAN-1 stably expressing miR-15a-5p was implanted subcutaneously in nude mice for in vivo ectopic tumorigenesis,and mice injected with untransfected CAPAN-1 cells were recorded as group A,mice injected with CAPAN-1 cells in the pcDNA3.1(+)empty vector group were recorded as group B,and mice injected with CAPAN-1 cells in the pcDNA3.1(+)-miR-15a-5p recom-binant plasmid group were recorded as group C.Mice injected with CAPAN-1 cells in the pcDNA3.1(+)-miR-15a-5p recombi-nant plasmid group were recorded as group C.1 cells were recorded as group C.The tumor volume was observed and recorded at 30 days.Tumor volume was observed and recorded,and the tumor tissues were removed after 30 days and weighed and recorded.Western blot assay was performed to detect the expression of miR-15a-5p and CCND1 in the grafted tumor tissues,and the grafted tumors were evaluated by applying tissue HE staining and fluorescence staining.Results Compared with the transplanted tumors of nude mice in Groups A and B,the volume and weight of transplanted tumors of nude mice in Group C were significantly lower(P＜0.05).Compared with the transplanted tumors of nude mice in groups A and B,the expression level of miR-15a-5p was sig-nificantly higher(P＜0.05)and the expression level of CCND1 was significantly lower(P＜0.05)in the transplanted tumors of nude mice in group C.In the transplanted tumor tissues of group A and B,the cells had hypertrophied nucleoli,more common nu-clear division,irregular morphology,unequal sizes,increased number,tight arrangement,dark color,and uneven distribution of coarse granular chromatin,and the cells of group C were sparsely arranged,with reduced density and increased necrotic area in the tissues compared with those of group A and B transplanted tumor tissues.The relative fluorescence intensity of CCND1 in the transplanted tumor tissues of group C was significantly lower compared with that of transplanted tumors in groups A and B(P＜0.05).Conclusion miR-15a-5p can regulate pancreatic cancer development and metastasis by modulating CCND1 and thereby regulating pancreatic cancer.
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维普
