Effects of miR-140-3p on the Proliferation,Invasion,and Migration Ability of Esophageal Squamous Cell Carcinoma Cells by Targeting CXCL8
Objective To investigate the effects of miR-140-3p on the proliferation,invasion,and migration ability of e-sophageal squamous cell carcinoma(ESCC)cells by targeting interleukin 8(CXCL8).Methods ESCC cell line EC109 cells were separated into:miR-NC group(transfected with miR-NC),miR-140-3p group(transfected with miR-140-3p mimic),miR-140-3p+CXCL8-NC group(co-transfected with miR-140-3p mimic and pcDNA-NC),miR-140-3p+CXCL8 group(co-transfected with miR-140-3p mimic and pcDNA-CXCL8),and EC109 cells without any treatment were recorded as NC group.Dual luciferase reporter gene experiment was applied to verify the relationship between miR-140-3p and CXCL8;qRT-PCR was applied to detect the expression of miR-140-3p in human EC109 cells and normal esophageal squamous cell line Het-1A;Western blot was applied to detect the levels of CXCL8 protein and epithelial mesenchymal transition(EMT)related proteins in Het-1A and EC109 cells;CCK8 method was applied to detect EC109 cell viability;flow cytometry was applied to detect the apoptosis rate of EC109 cells;Transwell was applied to detect the invasion and migration of EC109 cells.Results CXCL8 was highly expressed and miR-140-3p was low expressed in EC109 cells;compared with the NC group and miR-NC group,the OD450 value,migration and invasion cell counts,N-cadherin,vimentin,and CXCL8 protein levels of EC109 cells in the miR-140-3p group were obviously reduced(P＜0.05),the apoptosis rate,miR-140-3p level,and E-cadherin protein level of EC109 cells were obviously increased(P＜0.05),up-regulation of CXCL8 weakened the effect of overexpression of miR-140-3p on inhibiting EC109 cell proliferation,migration,in-vasion,EMT,and promoting apoptosis;miR-140-3p negatively regulated CXCL8 expression.Conclusion MiR-140-3p inhibits proliferation,migration,and invasion of ESCC cells by down-regulating CXCL8.
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