The Significance of Changes in Notch Pathway Related Proteins in Synovium and Synovial Fluid of Patients with Knee Osteoarthritis
Objective To investigate the changes and significance of Notch pathway related proteins(Notch1,Notch3,Jagged1,Jagged2,Hes5)in synovium and synovial fluid of patients with knee osteoarthritis(KOA).Methods 80 patients with KOA admitted to Dazu Hospital Affiliated To Chongqing Medical University from January 2021 to December 2022 were selected as the study object(KOA group),and 30 patients with meniscus injury admitted to our hospital in the same period were selected as the control group(control group).The severity of KOA was evaluated with Kelgren-Laurence(K-L)grading and Lequesne MG scale score.The expression levels of Notch1,Notch3,Jagged1,Jagged2,Hes5 in joint fluid and synovial tissue were detected by enzyme-linked immunosorbent assay and immunohistochemical staining respectively.The value of Notch1,Notch3,Jagged1,Jagged2,Hes5 in the diagnosis of KOA was analyzed with the subject's working characteristic curve(ROC).Results The expression levels of Notch1,Notch3,Jagged1,Jagged2,and Hes5 in the joint fluid and synovial tissue of the KOA group were significantly higher than those in the control group(P＜0.05).The higher the K-L grade,the greater the expression levels of Notch1,Notch3,Jagged1,Jagged2,and Hes5 in the synovial fluid and synovial tissue(P＜0.05).Additionally,the expression levels of Notch1,Notch3,Jagged1,Jagged2,and Hes5 in the synovial fluid and synovial tissue of KOA patients positively correlated with the Lequesne MG scale scores(P＜0.05).The AUC values for the diagnosis of KOA using Notch1,Notch3,Jagged1,Jagged2,and Hes5 in the joint fluid were 0.563,0.786,0.600,0.453,and 0.543,respectively,while the AUC for the combination of all five markers was 0.710.In synovial tissue,the AUC values for the same markers were 0.467,0.671,0.572,0.551,and 0.456,respectively,with a combined AUC of 0.787.When combining the markers in both joint fluid and synovial tissue,the AUC reached 0.845.Conclusion The Notch pathway in the synovium and synovial fluid of KOA patients exhibits abnormal changes.The detection of Notch pathway-related proteins,including Notch1,Notch3,Jagged1,Jagged2,and Hes5,is beneficial for diagnosing KOA and assessing the severity of the disease.
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