Establishment and Evaluation of a New Zealand Rabbit Model of Osteochondral Allograft
Objective To establish a New Zealand rabbit model of osteochondral allograft and evaluate its imaging and histological features.Methods A total of 36 5-month-old New Zealand rabbits,male or female,were selected.After treatment,part of the right tibial plateau osteochondral tissue was harvested as the allograft osteochondral donor in 12 rabbits.In the experimental group,5mm×5mm×5mm osteochondral defect were made,and the same amount of bone and cartilage tissue was grafted from osteochondral allograft and fixed on the tibial side of the knee joint of the right hind limb.Another 12 were for the control group,and the same amount of bone cartilage was taken at the same position on the tibial side of the knee joint of the right hind limb and fixed in situ.Bone healing was evaluated by X-ray after operation.Bone trabecular healing was evaluated by microCT.Histological changes were observed by staining of bone and joint sections.Results The animals in both groups survived well without postoperative complications.4 weeks after surgery,X-ray showed good bone healing.6 weeks after surgery,microCT showed good bone healing at the osteotomy.HE staining of joint sections showed good cartilage and subchondral bone structure,no significant difference in joint thickness between the two groups,no difference in Mankin score between the two groups,no difference in active chondrocyte count between the two groups.Conclusion The New Zealand rabbit knee osteochondral allograft animal model is simple to construct,strong operability,few surgical complications,and can better reflect the pathophysiological process of osteochondral allograft.
osteochondral allograftnew zealand rabbitanimal model
万方数据
维普
