Box-Behnken Design and Optimization of the Ultrafiltration Purification Process for Extracting Stachyose from Grass Silkworm
In order to improve the purity of stachyose,the small tangential flow ultrafiltration device is used to purify the stachyose extracted from the grass silkworm.Based on the single factor experiment,such as temperature,feed solution,pH of material and operating pressure,the ultrafitration process was opti-mized using response curves.The results show that the optimal process conditions obtained by response surface analysis are:temperature of 50.17 ℃,feed solution,pH of material liquid of 7.36,and operating pressure of 0.25 MPa.Under these conditions,the membrane efficiency is 33.96 g/(m2·min).After ul-trafitration the purity of stachyose in the extract of cordyceps sinensis reachs 68.87％which is 2.8 times higher than that before ultrafiltration,and highly fits to the model.
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