Box-Behnken设计优化草石蚕水苏糖提取液的超滤纯化工艺
Box-Behnken Design and Optimization of the Ultrafiltration Purification Process for Extracting Stachyose from Grass Silkworm
贺梦瑶 1任帅 1朱建星 1刘佳欣 1张万忠1
作者信息
- 1. 沈阳化工大学制药与生物工程学院,辽宁沈阳 110142
- 折叠
摘要
采用小型切向流超滤装置,对从草石蚕中提取的水苏糖进行初步纯化.在考察温度、料液pH、操作压力等单因素实验基础上,利用响应曲面对超滤工艺进行优化.结果表明:响应面分析法得到的最优工艺条件为:温度50.17 ℃、料液pH 7.36、操作压力0.25 MPa,在此条件下,膜效能为33.96 g/(m2·min),经超滤后草石蚕提取液中的水苏糖纯度可达到68.87％,是超滤前的2.8倍,与模型高度拟合.
Abstract
In order to improve the purity of stachyose,the small tangential flow ultrafiltration device is used to purify the stachyose extracted from the grass silkworm.Based on the single factor experiment,such as temperature,feed solution,pH of material and operating pressure,the ultrafitration process was opti-mized using response curves.The results show that the optimal process conditions obtained by response surface analysis are:temperature of 50.17 ℃,feed solution,pH of material liquid of 7.36,and operating pressure of 0.25 MPa.Under these conditions,the membrane efficiency is 33.96 g/(m2·min).After ul-trafitration the purity of stachyose in the extract of cordyceps sinensis reachs 68.87％which is 2.8 times higher than that before ultrafiltration,and highly fits to the model.
关键词
水苏糖/响应面/超滤Key words
stachyose/response surface/ultrafiltration引用本文复制引用
出版年
2024
NETL
NSTL
万方数据