摘要
目的 比较自动粪便分析仪与人工复核法两种粪便检测方法的检验效果.方法 收集2022年9 月—2023 年 8 月三明市第二医院就诊患者的 9402 份新鲜粪便标本,分别使用FA160 自动粪便分析仪和人工复核法检测粪便有形成分(白细胞、红细胞、脂肪球、真菌)和隐血,记录两种方法的检测结果并进行统计学分析.结果 自动粪便分析仪检测白细胞、红细胞、脂肪球、真菌和隐血的阳性检出率分别为 4.90%、2.67%、6.31%、4.54%、25.34%,人工复核法阳性检出率分别为 4.17%、2.22%、5.55%、5.29%、25.23%,自动粪便分析仪对粪便有形成分的阳性检出率除真菌略低于人工复核外,其他各项指标均高于人工复核,且差异均有统计学意义(均P<0.05),而粪便隐血试验的阳性检出率比较差异无统计学意义(P>0.05).以人工复核法为标准,自动粪便分析仪检测白细胞、红细胞、脂肪球、真菌、隐血的阳性符合率分别为 91.07%、96.17%、94.83%、78.07%、98.19%;阴性符合率分别为 98.85%、99.46%、98.90%、99.56%、99.25%;阳性预测值分别为 77.44%、80.08%、83.47%、90.87%、97.77%;阴性预测值分别为 99.61%、99.91%、99.69%、98.79%、99.39%.结论 自动粪便分析仪与人工复核法的检测结果虽然符合率较高,但还存在漏检和误检.因此对自动分析结果必须进行人工复核,提高检测结果的准确性,为临床提供可靠的参考依据.
Abstract
Objective To analyze the effect of automatic stool analyzer and manual review method in stool examination.Methods A total of 9402 fresh stool specimens from patients in the Second Hospital of Sanming City from September 2022 to August 2023 were collected and tested for fecal tangible components(white blood cells,red blood cells,fat globules and fungi)and occult blood using FA160 automatic stool analyzer and manual review method,respectively.The results of various parameters in stool examination were recorded for comparative analysis.Results The positive rates of white blood cells,red blood cells,fat globules,fungi and occult blood using automatic stool analyzer were 4.90%,2.67%,6.31%,4.54%and 25.34%,respectively.Comparatively,the positive rates of manual review method were 4.17%,2.22%,5.55%,5.29%and 25.23%,respectively.Except for fungi,which were detected by automatic stool analyzer with a lower positive rate of tangible components than that using manual review method,the positive rates of other parameters were higher,with statically significant differences(all P＜0.05).There was no significant difference in the positive rate of occult blood test(P＞0.05).The positive coincidence rates of positive specimen results were 91.07%for white blood cells,96.17%for red blood cells,94.83%for fat globules,78.07%for fungi,and 98.19%for occult blood,respectively.The negative coincidence rates were 98.85%,99.46%,98.90%,99.56%and 99.25%,respectively.The positive predictive values were 77.44%,80.08%,83.47%,90.87%and 97.77%,respectively.The negative predictive values were 99.61%,99.91%,99.69%,98.79%and 99.39%.Conclusions Although the results of stool samples detected by automatic stool analyzer have a high overall coincidence rate with the manual review method,the automated analyzer still exhibits instances of both missed detection and false detection.Therefore,it is necessary to check the results of automatic analysis manually to improve the accuracy of the test results,and to provide reliable references for clinical practice.
NETL
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万方数据