Objective To prevent the occurrence of discrepancies between urinary microalbumin(mALB)detection results and clinical actual situation due to hook-like effect,using bromocresol green method in combination with detection for specimens with hook-like effect,and to verify its feasibility.Methods Twenty fresh high-albumin urine samples were collected from the clinical laboratory department of Chongqing Armed Police Force Hospital from January 2023 to May 2023,and the theoretical concentration was determined according to the immunoturbidimetric method in original fold or dilution mode.Considering the low limit of detection and the upper limit of detection linear range of bromocresol green method,the concentration gradient of bromocresol green method was determined through increasing the sample by 5 times.The results of albumin determination with standard sample capacity of immunoturbidimetry and theoretical concentration were plotted to evaluate the hook-like effect.At the same time,bromocresol green method was used to detect the sample size increasing by 5 times,each sample was tested for 3 times,and the average value,coefficient of variation(CV)and deviation from the known theoretical concentration(Bias)were calculated.The feasibility of using bromocresol green method to detect samples with hook-like effect was evaluated.Results When the urinary mALB concentration was greater than 1 082 mg/L,the hook-like effect appeared by immunoturbidimetry,and the direct results were not consistent with the clinical results.When the concentration of urinary mALB reached 412 mg/L,the results of sample increment for 5 times could meet the clinical requirements.Conclusions When the concentration of urinary mALB reaches 412 mg/L,the bromocresol green method with 5 times increase of the sample could be used to replace the immunoturbidimetric method.The combined detection of the two methods could avoid the incompatibility of the detection results of urinary mALB due to hook-like effect.
关键词
尿微量白蛋白/钩状效应/免疫比浊法/溴甲酚绿法
Key words
Urinary microalbumin/Hook-like effect/Immunoturbidimetry/Bromocresol green method
维普
万方数据