Comparison on application effect of different molecular detection techniques in real world of acquired immune deficiency syndrome patients with parasitic infection
Comparison on application effect of different molecular detection techniques in real world of acquired immune deficiency syndrome patients with parasitic infection
Objective To preliminaryly detect the parasitic infection in hospitalized patients with acquired immune deficiency syndrome(AIDS)and compare the application effect of different DNA detection techniques on parasitic infection in AIDS patients in the real world.Methods The 16 patients with clinical diagnosis of AIDS and complicated with Toxoplasma gondii infection or Pneumocystis carinii infection were selected as research objects.The laboratory molecular testing was conducted using clinical diagnosis as"gold standard".The detection methods included fluorescence quantitative polymerase chain reaction(qPCR)and loop-mediated isothermal amplification(LAMP)for different target genes.The clinical samples included cerebrospinal fluid,plasma and bronchoalveolar lavage fluid(BALF).The detection results using different methods and clinical samples were compared.Results Using Rep-529 as target gene,the total detectable rates of LAMP and qPCR in diagnosing AIDS complicated with Toxoplasma gondii infection were both 53.12%,without statistically significant difference(P＞0.05).The detectable rate of LAMP-529-11 and qPCR-529 method in cerebrospinal fluid samples was significantly higher than that in plasma samples[90.62%(29/32)vs.15.62%(5/32),P＜0.01].The detectable rate of Pneumocystis carinii pneumonia in clinical diagnosis using BALF samples was significantly higher than that in plasma samples[100.00%(32/32)vs.18.75%(6/32),P＜0.01].The detectable rate of LAMP-18S was significantly higher than that of LAMP-Cob detection primer group[65.62%(21/32)vs.46.87%(15/32),P＜0.05].There was no statistically significant difference in the total detectable rate between LAMP-18S and qPCR-mtSSU[65.62%(21/32)vs.53.12%(17/32),P＞0.05].Conclusions When Rep-529 is used as the target gene to detect Toxoplasma gondii infection in AIDS patients,both qPCR and LAMP methods are optional,and it is recommended that the first sample is cerebrospinal fluid.qPCR and LAMP methods could also be used for detecting Pneumocystis carinii pneumonia,and it is recommended to choose BALF as the preferred sample.When choosing the LAMP method,it is recommended to prioritize primer sets targeting 18S.
维普
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