Objective To analyze the application of matrix-assisted laser desorption ionization time-of-flight mass spectrometry(MALDI-TOP MS)in the identification of Haemophilus influenzae.Methods The 1 300 samples of sputum or throat swab received in the laboratory of Nanjing Aidikang Medical Laboratory Co.,Ltd.from January to December,2022 were collected from respiratory infection patients,from which Haemophilus influenzae was cultivated and isolated.Using 16SrDNA sequencing,50 strains of Haemophilus influenzae were detected.The MALDI-TOP MS detection system,Analytical Products INC(API)biochemical bacterial identification system and paper method satellite experiment were used to identify Haemophilus influenzae and the detectable rate,detection time,detection cost and reading method of above three methods were compared.Results The detectable rate of Haemophilus influenzae by MALDI-TOP MS was 98.0%,the detectable rate of API biochemical bacterial identification system was 90.0%,and the detectable rate of paper disk satellite test was 74.0%.The detectable rate of Haemophilus influenzae by MALDI-TOP MS was higher than that of paper disk method satellite test,and the difference was statistically significant(P＜0.05).However,compared with API biochemical bacterial identification system,the difference was not statistically significant(P＞0.05).The time required for MALDI-TOP MS detection was 5-10 minutes,the time required for API biochemical bacterial identification detection was 4-24 hours,and the time required for paper disk satellite test detection was 24-48 hours.The cost of using MALDI-TOP MS to identify Haemophilus influenzae was lower,traditional API biochemical identification method was expensive,and the paper disk method satellite test operation was cumbersome.Conclusions The MALDI-TOP MS detection system has high accuracy,short identification cycle and low cost in identifying Haemophilus influenzae,and is easy to use.It has important value in the clinical detection of Haemophilus influenzae.
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