Objective To study the effectiveness of applying grading testing method in clinical blood lipid biochemical testing.Methods The 90 patients who underwent biochemical tests at the Third People's Hospital of Huangdao District,Qingdao City from January 2022 to December 2023 were selected as research subjects.Two samples of blood were collected from all patients,and routine screening test and grading testing method were used to detect blood lipid indicators,respectively.Total cholesterol(TC)and triacylglycerol(TG)were measured using enzymatic methods,low-density lipoprotein cholesterol(LDL-C)was measured using direct method surfactant method,high-density lipoprotein cholesterol(HDL-C)was measured using direct method selective inhibition method,and apolipoprotein A1(ApoA1)and apolipoprotein B(ApoB)were detected using immunoturbidimetry method.The test results and positive detectable rates obtained from two testing methods were compared,the test time and cost were recorded,and the missed detectable rates were calculated.Results The measured values and positive rates of LDL-C,ApoA1 and ApoB obtained through grading testing method were significantly higher than those obtained by routine screening test[LDL-C(mmol/L):4.50±0.62 vs.2.90±0.51;ApoA1(g/L):1.88±0.35 vs.1.32±0.22;ApoB(g/L):1.34±0.25 vs.0.86±0.13;positive rate of LDL-C:96.67%vs.70.00%;positive rate of ApoA1:24.44%vs.3.33%;positive rate of ApoA1:42.22%vs.0%;all P＜0.05].The testing time of grading testing method was significantly shorter than that of routine screening test(minutes:18.11±1.57 vs.23.75±3.69,P＜0.05),the testing cost was significantly lower than that of routine screening test(Yuan:30.44±2.80 vs.46.60±3.71,P＜0.05),and the missed detectable rate was significantly lower than that of routine screening test(4.44%vs.20.00%,P＜0.05).Conclusion Compared to routine screening test,grading testing method for blood lipid biochemical test has shorter detection time and higher accuracy.
维普
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