Characteristics of Microbial Community Construction in Soybean Continuous Cropping Rhizosphere Soil
To study the effects of continuous cropping on the composition and construction of bacterial and fungal communities in soybean rhizosphere soil,providing theoretical support for the biological control of soybean continuous cropping obstacles and soil remediation in farmland,experiment was conducted from 2021 to 2022 at the Tiexi District Positioning Test Station of Shenyang Agricultural University,with a type of brown soil area.The effects of continuous cropping on soybean soil chemical properties,soil enzyme activity,rhizosphere microbial diversity,and community construction characteristics were analyzed,taking maize soybean rotation(control)and continuous cropping of soybean rhizosphere soil as the research object,using 16S rDNA and ITS high-throughput sequencing technology,combined with soil chemical properties and enzyme activity measurements.The results showed that under continuous cropping,the content of soil chemical properties such as total nitrogen,total phosphorus,and total potassium decreased,while available potassium and pH increased.Continuous cropping significantly increased the activities of soil sucrase and catalase,while significantly reduced the activities of soil urease,leucine aminopeptidase,and N-acetylglucosaminidase.In continuous cropping mode,the soil bacterial diversity index(4.63)was lower than that of rotation treatment(5.34).At the bacterial level,continuous cropping increased the relative abundance of Sphingobacterium and Burkholderia genera(LRR:1.4%;LCR:2.4%);The soil fungal diversity index was lower than that of crop rotation treatment.At the fungal genus level,continuous cropping increased the relative abundance of pathogenic fungi Fusarium(LCR:20.5%;LRR:13.2%).In the process of community assembly,the construction of bacterial communities in the rhizosphere soil of soybean continuous cropping is mainly dominated by deterministic processes.The construction of bacterial communities in the rhizosphere soil of maize soybean rotation was mainly dominated by random processes.The construction of fungal communities in the rhizosphere soil of soybean continuous cropping and maize soybean rotation was dominated by random processes.Continuous cropping causes changes in soil environmental factors,bacterial and fungal community composition,and construction in the rhizosphere of soybean,leading to an increase in the relative abundance of pathogens.Among them,the increase in the relative abundance of Fusarium might be an important factor leading to the occurrence of soybean continuous cropping obstacles.
soybeanrhizospheric microorganismcommunity construction
万方数据
维普
