Identification of TPS Genes Family in Gardenia jasminoides and Its Relationship with Terpenoid Metabolism
Terpene synthase is a key enzyme in the synthesis of terpenoid compounds in Gardenia jasminoides and plays a key role in the formation of aromas.In order to clarify the basic characteristics of TPS Gene family members of G.jasminoides,the TPS gene family members of G.jasminoides were identified by bioinformatics method.We then used exogenous hormone spray test combined with transcriptomic approach to analyze the differential gene expression patterns of TPS gene family members in the flower of G.jasminoides.The contents of terpenoid compounds in flower of G.jasminoides at different developmental exogenous hormone spray were also measured by using head solid-phase micro-extraction combined with gas chromatography-mass spectrometry.The results showed that 41 members of GjTPS family were identified from genome database of G.jasminoides,encoding 380 to 849 amino acids and containing 5 to 15 exons.The members of GjTPS family were localized in chloroplasts and distributed unevenly on 10 chromosomes.Collinearity analysis revealed that TPS gene in G.jasminoides has a closer relationship with the TPS gene of Coffee canephora.Phylogenetic Analysis showed that GjTPS genes were divided into 5 subfamilies,and TPS-a and TPS-b subfamilies contained most of the members of GjTPS family.Most of cis-acting regulatory elements GjTPS promoters were in the Plant hormone response category,and the Methyl jasmonate Response element containing MYC motif is a large category among them.The transcriptome date showed that GjTPS02,GjTPS12,GjTPS14,GjTPS20,GjTPS21,GjTPS22 were highly expressed in all treatments.The correlation between gene expression and terpenoid content was analyzed,and it was found 1.25 mmol·L-1 methyl jasmonate contributes to gene expression of TPS family members and terpenoid release of G.jasminoides.GjTPS12,GjTPS14,GjTPS20,GjTPS21,GjTPS22 might be involved in the regulation of aroma formation in G.jasminoides.These result can provide reference for the subsequent function characteristics study of TPS gene family of G.jasminoides.
万方数据
维普
