Construction of Ziziphus jujuba var.spinosa Core Collection Based on Phenotypic Traits
In order to better preserve and study the germplasm of Ziziphus jujuba var.spinosa and improve the utilization efficiency,211 Z.jujuba var.spinosa germplasms were used as test materials in this study.Based on the phenotypic trait data,all germplasms were systematically clustered step by step at 25%sampling ratio.The best sampling strategy of Z.jujuba var.spinosa core collection was discussed from four aspects:genetic distance method,sampling method,clustering method and sampling ratio.According to the best sampling strategy and three grouping sampling methods,grouping sampling was carried out and compared with non-grouping sampling.The results showed that:Based on the 25%sampling ratio,the core collection constructed by the priority sampling method,Euclidean distance and variable class average method was the best.The overall sampling ratio was screened under the optimal construction strategy,and the results showed that the optimal overall sampling ratio was 25%.The variance difference percentage(VD),change rate of variation coefficient(VR)and range compliance rate(CR)of the core collection Pcore constructed by non-group sampling were higher than those of the core collection Core-L constructed by group sampling,and the construction effect was better.Qualitative description of trait retention ratio,t-test,coincidence rate test,principal component analysis and three-dimensional distribution of samples showed that the constructed core collections could eliminate genetic redundancy and represent the initial collection.The best construction strategy based on phenotypic traits was'Euclidean distance + variable class average method + priority sampling method + 25%sampling ratio'.After supplement and improvement,58 core collections were finally obtained,which were highly representative.This study successfully established the core collection that represented the phenotypic genetic diversity of initial Z.jujuba var.spinosa germplasm.This achievement contributes to scientific and effective collection and preservation of Z.jujuba var.spinosa germplasm resources,providing a scientific basis for exploring and utilizing Z.jujuba var.spinosa germplasm resources.Simultaneously,it lays the foundation for breeding Z.jujuba var.spinosa cultivar.
国家科技期刊平台
NSTL
万方数据
维普
