Analysis of the Current Status of Antibiotic Resistance Gene Contamination in the Soil of Japonica Rice Paddy Field in Shenyang
The issue of antibiotic resistance genes(ARGs)and mobile genetic elements(MGEs)pollution in agricultural soil has attracted widespread attention in the academic community.This study aimed to investigate the presence and correlation of ARGs and MGEs in paddy soil in Shenyang City's Japonica rice planting areas,and explore their potential impact on soil-plant systems.Soil samples were collected from Japonica rice planting areas in Liaozhong District,Xinmin City,Shenbei New District,and Sujiatun District of Shenyang City.High-throughput qPCR technology was used to detect and quantify 285 ARGs,10 MGEs and a 16S rRNA gene.The results showed that a total of 273 ARGs and MGEs were detected in paddy soil from different regions,with significant differences in species and abundance.Further correlation analysis revealed a certain correlation between ARGs and MGEs,which varied among different regions.Several ARGs[such as tet(32)]showed significant positive correlations with MGEs(such as tnp A-01),indicating that MGEs play a promoting role in the migration and diffusion of ARGs in soil.The total relative abundance of ARGs and MGEs in rhizosphere soil was higher than that in non-rhizosphere soil in all four sampling areas,highlighting the importance of rhizosphere environment in the distribution of ARGs and MGEs.Additionally,the study found that the antibiotic inactivation mechanism predominated in ARGs in paddy soil in different regions.In conclusion,this study provides data support and theoretical basis for understanding the pollution status and potential impact of ARGs in paddy soil in the northeast rice planting areas.These findings not only contribute to understanding the distribution and migration patterns of ARGs and MGEs in agricultural soil but also provide scientific basis for the development of targeted soil pollution prevention and control measures.
Japonica rice paddy soilantibioticsantibiotic resistance genesmobile genetic elements
万方数据
维普
