Genetic Diversity Analysis of Rice Blast Fungus in Different Regions of Northeast China Based on SSR
Rice blast is the main factor restricting the high and stable yield of rice.In order to clarify the genetic diversity of Pyricularia oryzae populations in Northeast region,96 strains isolated from Northeast region in 2021 were used as test materials.Simple sequence repeat(SSR)molecular marker technology was used to detect the tested strains of P.oryzae by PCR,and the genetic structure and genetic diversity of the tested P.oryzae populations were studied.The results showed that 16 pairs of SSR primers could amplify clear bands in 100‒500 bp.The 96 tested strains were divided into 13 genetic lineages.Among them,the DQL02 and DQL01 were the dominant lineages,including 37 and 22 strains,accounting for 38.54% and 22.92% of the total strains,respectively.DQL04,DQL06 and DQL08 were secondary lineages,including 9 strains,6 strains and 6 strains,accounting for 9.38%,6.25% and 6.25% of the total strains,respectively.The other 8 lineages were minor lineages,including 1‒4 strains.At the group level,the level of genetic diversity among populations was higher than that within populations,and the genetic variation within populations accounted for 83.37% of the total genetic variation.The average Nei's genetic diversity index among populations ranged from 0.083 6 to 0.457 1,with an average of 0.204 1.The average polymorphic percentage ranged from 6.25% to 100%,with an average of 45.67%,indicating that there were some differences in genetic diversity among the populations of P.oryzae in Northeast region.The results of cluster analysis showed that the genetic distance between the populations of P.oryzae in 13 regions in the Northeast was 0.011 0‒0.187 9,and there was a big difference.According to the genetic distance,the populations of P.oryzae in 13 regions in the Northeast could be divided into four groups.The second group was all composed of the populations of Liaoning Province,and the third group was all composed of the populations of Jilin Province,which reflected that the genetic pedigree of the population of P.oryzae in Northeast region was related to its geographical distribution.The results of this study can provide a theoretical basis for understanding the genetic structure and population evolution of P.oryzae populations in Northeast region.
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