Morphology and Inclusion Changes in the Development Process of Lycoris radiata Seed
To explore the morphological and inclusion changes during the development process of Lycoris radiata and determine the appropriate harvesting period,fresh L.radiata seeds on the 7,14,21,28,35,42,49,56,63 and 70 days after flowering(DAF)were used as research materials.Based on morphological observation,the moisture content and contents(soluble sugar,soluble protein,and starch)of the seeds were determined using drying method,coomassie brilliant blue G-250 method,and anthrone colorimetric method.The results showed that:the moisture content was still high when the seeds were mature and fell off(72.47%),seed viability(83.33%),soluble sugar(61.40 mg·g-1)and starch(35.65 mg·g-1)was high,while the content of soluble protein(0.70 mg·g-1)was very low,which was consistent with the characteristics of recalcitrant seeds.Based on the characteristics of seed morphological changes,its development process can be divided into five stages:vacuolization period(7-14 DAF),liquidization period(21-28 DAF),gelatinization period(35 DAF),embryonic development period(42-56 DAF),and maturity period(63-70 DAF),with a duration of approximately 70 days.During the entire developmental period,the color of the seed coat changes from initially translucent white to brown,and finally to black;The content of starch,soluble sugar,and protein in the endosperm significantly increases with the extension of development time,while the content of moisture content decreases significantly.Among them,the 42 DAF is an important node for the development of L.radiata seeds,and the embryo structure begins to appear.The embryo matured at 56-70 DAF,the germination rate were 8.67%,14.33%and 23.33%,respectively.Combined with the changes of internal and external morphological characteristics and inclusions of L.radiata seeds during the development process,it was determined that the appropriate harvest period was 63-70 DAF,that is,it should be harvested when the seed coat turned from brown to black and the moisture content was 72.47%-74.13%,when the seeds had completed dry matter accumulation and have germination power.
Lycoris radiatarecalcitrant seedseed developmentsoluble sugarstarchsoluble protein
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