Techniques on Leaf-explant Regeneration Based on Open Tissue Culture of Populus cathayana×canadansis Xinlin 1
In order to improve the efficiency and quality of poplar propagation,the Populus cathayana×canadansis Xinlin 1 were used as the object to study the types and concentrations of antimicrobial agents,inducing differentiation medium and rooting medium to establish the open tissue culture technique system.The leaves were used as the explant and sterilized with the low toxicity 75%alcohol,and the types and concentration of antimicrobial agents were screened with the chlorothalonil and the combination(chlorothalonil+sodium cefotaxime),and the bacterial inhibition system was established.The effects of 6-BA and NAA on adventitious bud induction were studied,and the effects of IBA on rooting was also studied.The results showed that the combination of chlorothalonil and cefotaxime sodium was effective as an antimicrobial agent and had less influence on the growth and differentiation of the open tissue culture of the Populus cathayana×canadansis Xinlin 1.The suitable inhibition concentration was 0.15 g·L-1 chlorothalonil+0.05 g·L-1cefotaxime sodium.The suitable differentiation medium was 0.2 mg·L-1 6-BA+0.04 mg·L-1 NAA,the induction rate was(91.57±0.21)%,and the average number of adventitious buds was 7.5±1.0.The suitable rooting medium was 1/2MS+IBA 0.3 mg·L-1.and the transplanting survival rate was over 95%.The open tissue culture technique system of the Populus cathayana×canadansis Xinlin 1 was preliminarily established,providing a technique support for the rapid propagation of open tissue culture of Populus cathayana×canadansis Xinlin 1,and also laying the foundation for the large-scale factory propagation of poplar cultivars.
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