Analysis and Comprehensive Evaluation of Quality Traits of Dry Direct-seeded Japonica Rice Germplasm Resources in Northeast China
A systematic evaluation of the quality traits diversity of dry direct-seeded rice germplasm resources provides a theoretical basis for the innovation of rice germplasm resources and quality breeding.Using 79 japonica rice germplasms as materials,genetic diversity analysis and correlation analysis were conducted on the processing quality,appearance quality,nutritional quality,cooking and eating quality,and starch pasting characteristic parameters of rice.Principal component analysis,membership function method,and cluster analysis were used to comprehensively evaluate the quality of rice varieties and classify genotype differences.The experimental results showed that the dry direct-seeded rice germplasm resources exhibited relatively rich variation in multiple quality traits,with the coefficient of variation of the measured traits ranging from 1.63%to 62.69%,among which the coefficient of variation of chalkiness degree was the largest.Correlation analysis revealed significant correlations among various quality indicators and between quality indicators and starch pasting characteristic parameters.Four principal components were extracted using principal component analysis,with contribution rates of 38.272%,17.063%,12.188%,and 8.297%,respectively,and a cumulative contribution rate of 75.819%.Based on the feature vectors of each principal component and the factor loadings of each trait indicator,the comprehensive quality evaluation D value was calculated and subjected to cluster analysis,categorizing the tested materials into three types:high-quality,medium-quality,and low-quality.Accordingly,the key indicators for identifying the quality of direct-seeded rice were selected as head rice rate,amylose content,peak viscosity,trough viscosity,and final viscosity.The correlation analysis results between each quality indicator and the comprehensive evaluation D value further confirmed the representativeness of the selected indicators.In summary,the comprehensive evaluation D value can accurately and objectively evaluate the overall quality of dry direct-seeded japonica rice.