Isolation and Indentification of Higher Yield EPS and Its Application in the Improvement of Arid Region Residue
This study explores the soil improvement potential of high-yield exopolysaccharide(EPS)strains for waste soil,providing new remediation strategies and microbial resources to address the shortage of topsoil.Three high-yield EPS-producing soil bacteria were isolated in situ from waste soil:Peribacillus frigoritolerans,Bacillus cereus and Bacillus velezensis.These strains were used as composite inoculants in pot experiments under drought stress,with soil-to-waste ratio,particle size distribution,inoculant concentration,and inoculant dosage as orthogonal test factors.Principal component analysis was employed to optimize the substrate formulation,resulting in the optimal remediation ratio:nutrient soil-to-waste soil=7∶3,particle size distribution=5 mm,inoculant concentration=0.6(OD600),and inoculant dosage=150 mL·kg-1.The application of high-yield EPS inoculants was found to be an effective strategy for improving waste soil in arid regions.However,its efficiency is influenced by soil texture and structural characteristics.EPS-producing inoculants significantly improved the structure,physicochemical properties,and enzyme activity of waste soil,helping plants resist drought stress.These findings highlight the strong potential of EPS inoculants for waste soil remediation.
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