Study on the Effect of Preservation Environments on the Trehalose Content of Antheraea pernyi Pupae and Related Mechanisms
Trehalose,as the blood sugar in tussah(Antheraea pernyi),can form a stable complex with protein,protect the body protein from damage during the overwintering period,and participate in nutrient storage and metabolism,which is of great significance for the physiological process of diapause and overwintering of Tussah In this study,we measured trehalose content,related enzyme activity,and gene expression of tussah silkworm pupae after the termination of diapause,and compared the effects of different preservation temperatures on trehalose content of tussah overwintering pupae,aiming to increase the selection pressure of breeding by changing the environmental conditions,and to obtain breeding materials with higher trehalose content.The results showed that the trehalose content of"J"material in a low-temperature preservation environment was higher than that of conventional material,which indicated that the trehalose content of"J"material was more affected by temperature change.In contrast,the change of"D"material was not obvious,the trehalose content of male pupae was higher than that of female pupae.Both materials showed higher trehalase activity in the cold environment than in the conventional environment.The"D"material showed that trehalase activity in male pupa was higher than that in female pupa under different preservation environments.On the contrary,the"J"material showed that female pupae were larger than male pupae under different preservation conditions.The expression of four genes,ApTreh1A,ApTreh1B,ApTreh2,and ApTPS,showed that the expression of ApTreh1A in male pupae and ApTreh1B in female pupae of"D"material in comparison with other treatments under the cold environment.The differences of other genes were insignificant,but the relative expression of"D"was higher.In conclusion,changing the preservation temperature could affect the content of trehalose and other substances,and achieve the purpose of improving the breeding selection pressure which is conducive to screening the materials with high trehalose content and the form of parental combinations,and providing technical assistance for the breeding of new varieties of tussah.
国家科技期刊平台
NSTL
万方数据
