Expression and targeting relationship of miR-557 and eIF2a in keloid
Objective To detect the expression levels of microRNA-557(miR-557)and eukaryotic translation initiation factor 2a(eIF2a)in keloid tissue and cell lines,and analyze their targeting relationship.Methods Keloid tissues(98 cases),hypertrophic scar tissues(49 cases)and normal skin tissues(49 cases)were collected from patients who underwent treatment at Beijing Chaoyang Hospital.The expression of miR-557 was quantified using real-time quantitative fluorescence PCR(qRT-PCR),while the expression of eIF2a and Ki-67 was detected through immunohistochemistry(IHC).Fibroblasts derived from keloid tissues were isolated and cultured,followed by the assessment of the targeting relationship between miR-57 and eIF2a by dual luciferase reporter assay.Results The expression of miR-557 was significantly lower in keloid tissues compared to that in hypertrophic scar tissues and normal skin tissues(P＜0.05).The expression of eIF2a was significantly higher in keloid tissues compared to that in hypertrophic scar tissues and normal skin tissues(P＜0.05).The expression of miR-557 and eIF2a exhibited significant variations across different maximum diameter,proliferation index and accompanied symptoms(P＜0.05).Negative correlation was found between miR-557 and eIF2a in keloid tissues(r=-0.69,P=0.0 16).Targeting relationship was found between miR-557 and eIF2a by dual luciferase reporter assay.Conclusions The expression of miR-557 was downregulated,while the expression of eIF2a was upregulated in keloids,which is associated with clinical and pathological features.Negative targeting regulatory relationship was found between miR-557 and eIF2a in keloids.
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