Screening and validation of microRNA related to the regulation of Rab23 and the prognosis of head and neck squamous cell carcinoma
Objective The screening and validation of MicroRNA(miRNA)related to the regulation of Rab23 and the prognosis of head and neck squamous cell carcinoma(HNSCC)was conducted through bioinformatics method,aiming to explore underlying mechanisms in development and invasion of HNSCC,thereby offering novel research perspectives and methodologies.Methods The screening of the potential miRNAs regulating Rab23 expression were performed using the miRWalk database,and the differentially expressed miRNAs associated with prognosis of HNSCC were obtained from both the TCGA public database and the miRWalk database.The miRNA related to the regulation of Rab23 and the prognosis of HNSCC was identified by overlapping candidate miRNAs of two groups.The results were subsequently validated using RT-qPCR,Western-Blot,and double luciferase reporter assay.Results A total of 250 differentially expressed miRNAs were identified,including eight miRNAs involved in the regulation of Rab23.The hsa-miR-363-3p was related to the regulation of Rab23 expression,as well as the prognosis of HNSCC.The negative regulation between hsa-miR-363-3p and Rab23 in tumor cells and transfected cell models was confirmed by RT-qPCR and Western-Blot assay.The subsequent double luciferase reporter assay confirmed the binding of hsa-miR-363-3p to Rab23.Conclusion The hsa-miR-363-3p involves in the targeted regulation of Rab23 expression and likely plays a role in the invasion and metastasis of HNSCC.
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