摘要
目的 调查胆道闭锁患儿在肝移植术后的生长发育情况及其肠道微生态的现状,并分析两者的相关性.方法 采集30例肝移植患儿的粪便标本,采用16SrRNA基因测序法对肠道菌群进行高通量测序分析,分别测定术前及术后14 d的患儿粪便标本.同时收集患儿的临床资料及个人信息,进行统计分析.结果 患儿的平均月龄为(6.1±1.5)m,平均身长为(65.8±3.7)cm,平均体重为(6.7±1.1)kg,平均体重指数(body mass index,BMI)为 15.1±1.6.性别:男性 10 例(33.3％),女性 20 例(66.7％);血型:A 型 10 例(33.3％),B型7例(23.3％),O型9例(30.0％),AB型4例(13.4％).手术当天,生长发育正常组和异常组患儿的体重、BMI、体重Z值以及BMIZ值的差异有统计学意义,且各项数据均为正常组高于异常组.而在术后18个月时,两组患儿的身长和体重的差异有统计学意义,且两项数据均为正常组高于异常组.两组患儿手术时的肠道菌群门和属差异均有统计学意义,术后14 d时的肠道菌群的属差异有统计学意义,门差异没有统计学意义.生长发育正常组患儿术前术后肠道菌群的门和属差异均有统计学意义.生长发育异常组患儿术前术后肠道菌群的门和属差异均有统计学意义.结论 肝移植术能改善胆道闭锁患儿的生长发育,肠道菌群和生长发育有相关性.
Abstract
Objective This survey was conducted to get some knowledge about the growth status and intestinal microecology of pediatric liver transplantation patients.Methods A total of 30 children's stool sample were collected,the samples were analyzed by gene sequencing.Meanwhile,the clinical data and personal information were included into analysis.Results The average month age was(6.1±1.5)m.The average height,weight and body mass index(BMI)were(65.8±3.7)cm,(6.7±1.1)kg and 15.1±1.6,respectively.As for the gender,10 cases were men while 20 cases were women.The most common blood type was A which accounted for 33.3％,followed by B(23.3％),O(30.0％)and AB(13.4％).There were statistical differences between normal and abnormal groups in weight,BMI,weight Z score and BMI Z score on the operation day.However,18 months after operation,there were statistical differences between two groups in height and weight.Conclusion Liver transplantation can improve the growth of biliary atresia patients,and intestinal flora correlates to growth.
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