间充质干细胞改善长期培养人胰岛功能的体外研究
The in vitro research on hUC-MSCs to promote the function of long-term cultured human islets
郑琳怡 1冯淑怡 1宋益康 1林悦 1傅红兴2
作者信息
- 1. 浙江树人学院树兰国际医学院,浙江 杭州 310015
- 2. 浙江大学医学院附属第二医院,浙江 杭州 310003
- 折叠
摘要
目的 探讨人脐带间充质干细胞(human umbilical cord mesenchymal stem cells,hUC-MSCs)对长期培养的人胰岛功能的改善作用.方法 将hUC-MSCs根据不同浓度分成 3 组:104 组、105 组、106 组,每组平行三孔接种于 12 孔板中,经培养 24 h贴壁后,各孔分别加入 150 IEQ培养了 17 d的人胰岛,以无hUC-MSCs的胰岛培养为对照组.经 37℃,5%CO2 培养 24 h后,比较不同组胰岛外形、活性和静态葡萄糖刺激胰岛素释放指数(insulin release index,GSI).结果 培养后,各组胰岛形态紧实,106 组培养液有较多细胞成膜片漂浮,105 组胰岛活性显著高于对照组胰岛(P＜0.01),106 组胰岛活性相对较差(P＜0.01).105 组胰岛的葡萄糖刺激GSI与对照组具有显著性差异(3.63±0.16 比 2.52±0.10,P＜0.01),其余两组人胰岛GSI与对照组无显著性差异.结论 hUC-MSCs可以改善长期培养后人胰岛细胞团的体外活性和功能,但与MSCs细胞浓度有关.
Abstract
Objective To investigate the promoting effect of human umbilical cord mesenchymal stem cells(hUC-MSCs)on long-term cultured human islets.Methods The hUC-MSCs were divided into three groups according to different cell concentrations:group 104,group 105 and group 106.Each group was inoculated into 12-well plates in three parallel wells.After culturing for 24 h,150 IEQ human islets cultured for 17 d were added to each well and the group cultured without hUC-MSCs was a control.After culturing at 37℃and 5%CO2 for 24 h,the islet shape,activity and static glucose-stimulated insulin release index(GSI)were compared in different groups.Results After culture,the shapes islets in each group were compact,and there were some dead cells floating in the culture medium of group 106.The activity of islets in group 105 was significantly higher than that in the control group(P＜0.01),and the activity of islets in group 106 was relatively poor(P＜0.01).The glucose stimulation GSI of pancreatic islets in group 105 was significantly higher than that of the control group(3.63±0.16 vs.2.52±0.10,P＜0.01).There was no significant difference in the GSI of human pancreatic islets between the other two groups and the control group.Conclusion hUC-MSCs can improve the in vitro activity and function of human pancreatic islet cell mass after long-term culture,but it is related to the concentration of MSCs.
关键词
人脐带间充质干细胞/人胰岛/长期培养/功能Key words
Human umbilical cord mesenchymal stem cells/Human pancreatic islets/Long-term culture/Function引用本文复制引用
基金项目
浙江树人学院大学生创新创业训练计划项目(202311842046X)
出版年
2024
NETL
NSTL
万方数据