离体肝脏长期灌注系统的构建
Establishment of a long-term Ex situ liver perfusion system
刘之颖 1陈宏珲 1李冶夫 1陈志涛 1杨永琪 1赵强 1何晓顺1
作者信息
- 1. 中山大学附属第一医院器官移植中心,广东 广州 510080
- 折叠
摘要
目的 使用常温机械灌注(normothermic machine perfusion,NMP)建立离体肝脏长期灌注系统(ex situ MP for long-term liver perfusion).方法 将 1 例子猪全肝连接至Life-X100 灌注系统进行常温机械灌注,持续监测灌注过程中灌注参数及灌注液血气分析结果,评估离体肝脏的功能.结果 成功离体灌注猪肝 138 h,各项灌注参数稳定,离体肝脏功能维持良好.结论 我们初步建立了离体肝脏长期灌注系统,在未来可用于人类肝脏的离体养护,并在此基础上进行多项探索性研究,促进我国医学发展.
Abstract
Objective To establish an ex situ machine perfusion(MP)system for long-term liver perfusion using normothermic machine perfusion(NMP)technology.Methods A porcine liver was connected to the Life-X100 perfusion system for normothermic machine perfusion.Perfusion parameters and perfusate blood-gas analysis results were continuously monitored during the perfusion process to assess the ex situ liver function.Results The porcine liver was successfully perfused ex situ for 138 hours,with stable perfusion parameters and well-maintained liver function.Conclusion We have preliminarily established an ex situ long-term liver perfusion system.This system can be used for the ex situ maintenance of human livers in the future and can serve as a basis for various exploratory studies to advance medical development in our country.
关键词
长期常温机械灌注/离体肝脏保存/器官功能维护/器官医学Key words
Long-term normothermic machine perfusion/Preservation of liver in vitro/Organ function maintenance/Organ medicine引用本文复制引用
出版年
2024
NETL
NSTL
万方数据