CD4+CD25+调节性T细胞及TGF-β1在肺炎支原体肺炎患儿中的表达和意义
Significance and Expression of CD4 + CD25 + regulatory T cells and TGF-β1 in children with mycoplasma pneumoniae pneumonia
刘小群 1陈兰举 2陈啸洪 1张佩红1
作者信息
- 1. 312000 浙江省绍兴市人民医院(浙江大学绍兴医院)小儿内科
- 2. 233004 安徽省蚌埠医学院第一附属医院儿内科
- 折叠
摘要
目的 初步探讨CD4+ CD25+调节性T细胞(Treg)及转化生长因子-β1(TGF-β1)在肺炎支原体肺炎(MPP)发病中的作用及可能机制.方法 采用流式细胞术(FCM)和ELISA法分别检测2组小儿(MPP组及健康对照组)外周血CD4+ CD25+ Treg细胞的比例及血清TGF-β1水平,并分析二者之间的相关性.结果 ①MPP组患儿外周血中CD4+ CD25+ Treg细胞比例和血清TGF-β1水平分别为[(5.59％±1.18％)和(467.74±142.05) ng/L],与健康对照组[(8.20％±1.76％)和(730.05±197.21) ng/L]相比,均显著降低(P＜0.01);②MPP组小儿血清TGF-β1水平与CD4+ CD25+ Treg细胞比例呈正相关(r=0.71,P＜0.01).结论 CD4+ CD25+ Treg细胞比例及TGF-β1水平的降低可能参与MPP的发病.
Abstract
Objective To explore the role and possible mechanisms of CD4 + CD25 + Treg and TGF-β1 in Pathogenesis of Mycoplasma pneumoniae pneumonia.Methods MPP and control group of CD4 + CD25 + Tregs' ratio were detected by flow cytometry,while serum TGF-β1 levels were determined by Enzyme-linked immuneosorben,and analyze correlation of the two.Results ①The ratio of CD4+ CD25 + Tregs and serum TGF-β1 levels in MPP group were(5.59％ ± 1.18％) and (467.74 ± 142.05) ng/L,which were significantly lower than those of control group [(8.20％ ± 1.76％) and (730.05 ± 197.21) ng/L] (P ＜ 0.01);②The serum TGF-β1 levels and CD4 + CD25 + Tregs' ratio of the MPP group were concentration positively correlated(r =0.71,P ＜ 0.01).Conclusion The reduction of CD4 + CD25 + Tregs' ratio and serum TGF-β1 levels could have close ties with the pathogenesis of MPP.
关键词
CD4+/CD25+调节性T细胞/TGF-β1/小儿/肺炎支原体/肺炎Key words
CD4+ CD25+ regulatory T cells/TGF-β1/Children/Mycoplasma pneumoniae/Pneumonia引用本文复制引用
出版年
2015
NETL
NSTL
万方数据