鼻咽吸出物IL-4、IL-12和IFN-γ在婴幼儿喘息中的变化及意义
Changes of IL-4 ,IL-12 and IFN-γ levels in nasopharyngeal aspirates of infants with wheezing
姚欢银 1张佩红 1李华浚 1王小仙 1刘淑梅 1陈啸洪1
作者信息
- 1. 绍兴市人民医院(浙江大学绍兴医院)儿内科,浙江 绍兴 312000
- 折叠
摘要
目的 通过测定婴幼儿喘息患儿鼻咽吸出物中细胞因子IL-4、IL-12和IFN-γ的水平变化,探讨婴幼儿喘息与机体细胞因子反应类型的关系.方法 应用固相夹心ELISA法测定和比较58例婴幼儿喘息和47例无喘息的婴幼儿肺炎鼻咽吸出物IL-4、IL-12和IFN-γ水平变化,同时对急性期和恢复期婴幼儿喘息鼻咽吸出物IL-4、IL-12和IFN-γ水平进行测定和比较.结果 与婴幼儿肺炎组[(275.11±30.27)ng/L]、对照组[(253.76±39.9) ng/L]和婴幼儿喘息恢复期组[(260.88±28.07)ng/L]比较,鼻咽吸出物IFN-γ水平在婴幼儿喘息组急性期[(142.55±26.56) ng/L]明显下降(P＜0.01),而IL-4水平[分别为(8.51±2.02)ng/L,(3.14±1.16) ng/L,(4.95±1.93) ng/L,(3.28±1.36) ng/L)]和IL-4/IFN-γ(分别为0.068 ±0.044,0.012±0.004,0.019±0.007,0.014±0.005)比值则明显升高(P＜0.05);IL-12在婴幼儿喘息急性期组[(587.24±58.14) ng/L]明显低于肺炎组[(1 322.13±139.74) ng/L](P＜0.01),与恢复期组[(580.77±64.32)] ng/L和对照组[(604.02±88.38)ng/L]比较差异无统计学意义(P＞0.05).结论 婴幼儿喘息急性期局部主要表现为Th2型细胞免疫反应,鼻咽吸出物IL-4产生明显增加,IFN-γ水平则明显下降,而IL-12在婴幼儿肺炎中产生增加.
Abstract
Objective To test the levels of IL-4,IL-12 and IFN-γ in nasopharyngeal aspirates (NPS) of infants with wheezing,and to explore the relationship between the infantile wheezing and the type of cytokine response.Methods The NPS of IL-4,IL-12 and IFN-γlevels were measured by ELISA and compared in 58 cases of acute stage and convalescent stage with infantile wheezing and 47 cases of infantile pneumonia.Results The NPS of IFN-γ levels in acute group [(142.55±26.56) ng/L] were significantly lower than (275.11±30.27) ng/L of the infantile pneumonia group,(253.76±39.9) ng/L in the control group and (260.88±28.07) ng/L in the recovery group,P ＜ 0.01;while the IL-4 [(8.51±2.02) ng/L,(3.14±1.16) ng/L,(4.95±1.93) ng/L,(3.28±1.36) ng/L] and the ratio of IL-4/IFN-γ (0.068±0.044,0.012±0.004,0.019±0.007,0.014±0.005) were significantly higher (P＜0.05);Compared with the infantile pneumonia group[(1322.13±139.74) ng/L],the acute group' s IL-12 level[(587.24±58.14) ng/L] in NPS were significantly lower(P＜0.01) and no statistical difference with the recovery group [(580.77±64.32)ng/L]and the control group[(604.02±88.38)ng/L],P ＞ 0.05.Conclusion The acute Stage of wheezing in infants are with the Th2-predominant response,increase of IL-4 level,decrease of IFN-γ level in NPS,and IL-12 increase in infantile pneumonia.
关键词
鼻咽吸出物/婴幼儿喘息/IL-4/IL-12/IFN-γKey words
Nasopharyngeal aspirates/Wheezing in infants/IL-4/IL-12/IFN-γ引用本文复制引用
基金项目
浙江省医药卫生一般研究计划项目(2015-KYA219)
出版年
2016
NETL
NSTL
维普
万方数据