Objective To detect the expression of cluster of differentiation 38(CD38)and the ratio of regulatory T cells(Tregs)to helper T cells 17(Th17)among CD4+T cells in the tissues of rheumatoid arthritis(RA)mouse model,and to explore the mechanism by which CD38 in promoting the development of rheumatoid arthritis.Methods The collagen-induced mice arthritis model(CIA)were constructed to detect CD38 protein and mRNA expression in synovial tissue,spleen,and lymph nodes by protein immunoblotting and fluorescent quantitative PCR.The ratio of Th17 cells and Treg cells were analyzed by flow cytometry.After differentiation,naive CD4+T cells isolated from the spleen of mice were test-ed for CD38 protein expression and the ratio of Th 17 cells to Treg cells was calculated;By polarizing naive CD4+T cells,the protein expression of PI3K,AKT,p-AKT,mTOR,p-mTOR were detected and the effect of CD38 on PI3K/AKT/mTOR signaling pathway were analyzed.Results CD38 protein expression in synovial tissue,spleen,and lymph nodes in CIA were higher than that in the control group,and the differences were statistically significant(P＜0.01).The per-centage of naive CD4+T cells in specific differentiation conditions,compared with the polarized group+sh-NC,the pro-portion of Th17 cells in the polarized group+sh-CD38 was decreased,and the proportion of Treg cells was increased(P＜0.01).After the polarization treatment of CD4+T cells,the expression of p-AKT and p-mTOR proteins in the polarized group+sh-NC(3.00±0.08,3.18±0.12)were higher than that in polarized group+sh-CD38(2.48±0.09,1.70±0.10,P＜0.01).Conclusion CD38 is highly expressed in CIA,and inhibition of CD38 expression improves CIA inflamma-tion.Under specific differentiation conditions,high CD38 expression increases the proportion of Th17 cells and decreases the proportion of Treg cells.Through polarization,CD38 affects the balance between Treg and Th17 through the PI3K/AKT/mTOR signaling pathway to promote the development of rheumatoid arthritis inflammation.
Cluster of differentiation 38Signal pathwayRegulatory T cells/T helper type 17 balanceRheumatoid ar-thritis
维普
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