Objective To detect the percentage of CD22 in peripheral blood lymphocytes of healthy adults by flow cytom-etry.The reference range for healthy adults was established according to the requirements of Clinical and Laboratory Standards Institute EP28-A3c.Methods Peripheral blood was collected from healthy people who underwent physical ex-amination in Peking University People's Hospital and the First Affiliated Hospital of Bengbu Medical University from July to November 2022.The peripheral blood CD22 levels of 130 healthy adults were measured by flow cytometry,and the da-ta of each group were grouped according to region,sex and age according to the grouping requirements in the EP28-A3c file,so as to establish the corresponding biological reference interval.Results The level of CD22 in peripheral blood of healthy adults was normally distributed,and the positive rates of CD22 in peripheral blood of healthy adults in Beijing and Anhui were(10.87±4.37)％and(12.62±4.03)％,respectively,with no statistical significance(t=1.336,P=0.205).The positive rates of CD22 in male and female peripheral blood were(11.76±4.86)％and(11.85±3.69)％,respectively,and the difference was not statistically significant(t=0.111,P=0.911).They were divided into ≤35 yearsold group,36-60 years old group and＞60 years old group.The positive rate of CD22 in peripheral blood of the three groups were(11.53±3.20)％,(11.61±3.80)％and(12.73±6.36)％,respectively,and there was no statistical significance(F=0.761,P=0.469).According to the 90％confidence interval recommended by the International Com-mittee for Clinical Laboratory Standards,the reference intervals of CD22 in lymphocytes(flow cytometry)was 4.82％-18.80％.Conclusion The reference intervals of CD22 positive rate in peripheral blood of healthy adults are consistent in different regions,gender and age.It can be combined to establish the reference intervals of CD22 positive rate in pe-ripheral blood of healthy adults.
维普
万方数据
