Objective To investigate the regulatory effects of miR-206 on senescence and apoptosis of nucleus pulposus cells in white rabbits with lumbar disc herniation,and to provide a reference for targeted therapy of lumbar disc hernia-tion.Methods Thirty cases of New Zealand white rabbits were selected as the study subjects to construct white rabbit model of lumbar disc herniation by autologous nucleus pulposus transplantation.The rabbits were randomly divided into a model group and a control group,with 15 rabbits in each group.The rabbits in the model group were injected with miR-206 simulant,and the rabbits in the control group were injected with normal saline.The transcription level of miR-206 was detected by reverse transcription-polymerase chain reaction(qRT-PCR).Senescence of nucleus pulposus cells was detected by senescence-associated β-galactosidase(SA-β-gal).Nucleus pulposus apoptosis was detected by flow cytome-try.Results The expression level of miR-206 in nucleus pulposus cells in the model group(1.65±0.32)was signifi-cantly higher than that in the control group(0.41±0.08),the difference was significant(P＜0.05).The number of pos-itive cells in the model group[(14.27±5.36)×105]was higher than that in the control group[(5.08±1.13)×105],the difference was statistically significant(P＜0.05).The apoptosis rate of nucleus pulposus cells in the model group[(8.18±1.94)％]was lower than that in the control group[(42.43±6.28)％],the difference was statistically signifi-cant(P＜0.05).Conclusion miR-206 plays an important regulatory role in nucleus pulposus cells of white rabbits with lumbar disc herniation.Overexpression of miR-206 can reduce the senescence of nucleus pulposus cells,decrease the ap-optosis rate of nucleus pulposus cells in white rabbits with lumbar disc herniation,and improve the proliferation ability of nucleus pulposus cells.
miR-206Lumbar disc herniationThe big white rabbitNucleus pulposus cellsAgingApoptosis
维普
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