Objective To investigate the value of conventional ultrasound versus ultrasonography as a guide in biopsy of peripheral lung-type lesions of different sizes.Methods A total of 361 patients with peripulmonary lesions were collect-ed from the First Affiliated Hospital of Bengbu Medical University from January 2022 to July 2023,and all of them under-went routine ultrasound or ultrasound-guided lung puncture biopsy,and were divided into three groups of A,B,and C according to the maximum diameter of the lesions(Group A:≤20 mm;Group B:21-49 mm;Group C:≥50 mm),and the lesions of the three groups were further divided into the routine group and the contrast-enhanced ultrasound(CEUS).The lesions in the three groups were further divided into the conventional group and the CEUS group.The de-tection rate of internal necrotic areas,the detection rate of lesions and surrounding large vessels,the success rate of punc-ture,the number of punctures and the complication rate were compared between the conventional group and the CEUS group at different sizes.Results The differences in baseline characteristics such as gender and age among the three groups were not statistically significant(P>0.05).In group A,there was no significant difference in the above biopsy in-dices between the conventional group and the CEUS group.In group C,the display rate of internal necrotic area(70.6%,48/68),the display rate of lesions and peripheral vessels(47.1%,32/68)and the success rate of puncture(97.1%,66/68)were significantly higher in the CEUS group than in the conventional group[17.1%(14/82),20.7%(17/82),87.8%(72/82)],with statistically significant differences(P<0.05).Conclusion Ultrasound-guided puncture biopsy is more informative when the maximum diameter of the lesion is≥50 mm;routine ultrasound-guided puncture biopsy is more recommended for smaller peripulmonary lesions(≤20 mm)to save patient's cost and medical resources.