Objective To investigate circular RNA is associated with the occurrence and development of non-small cell lung cancer.This study aims to investigate the mechanism of circ0006152/miR-557/Wnt3a signaling axis in the occur-rence and development of non-small cell lung cancer A549 cells.Methods Take A549 cells in logarithmic growth peri-od for grouping and transfection,and divide them into control group(cells are only routinely cultured),si-NC group(transfected with si-NC),si-circ0006152 group(transfected with si-circ0006152),miR-NC group(transfected with miR-NC),miR-557 mimics group(transfected with miR-557 mimics)and si-circ0006152+miR-557 inhibitors group(transfected with miR-557 inhibitors and si-circ0006152)by random number table method.Proliferation,apoptosis,mi-gration invasion and Wnt3a,beta-catenin protein were detected.Results Compared with the si-NC group,the prolifera-tion inhibition rate and apoptosis rate of A549 cells in the si-circ0006152 group were significantly increased,and the number of invasion was significantly decreased(P＜0.001);Compared with the miR-NC group,the proliferation inhibi-tion rate of A549 cells[(48.42±5.73)％vs.(4.27±0.19)％]and apoptosis rate of A549 cells in miR-557 mimics group[(45.38±5.96)％vs.(8.94±2.11)％]were significantly increased.The number of cells invaded(55.42±8.65 vs.167.42±19.53)was significantly decreased(P＜0.001).The cell proliferation inhibition rate and apoptosis rate in the si-circ0006152+miR-557 inhibitors group were significantly decreased,and the number of cell invasion was signifi-cantly increased in the si-circ0006152+miR-557 inhibitors group(P＜0.05).The expressions of Wnt3a and β-catenin proteins in the inhibitors group were significantly increased(P＜0.05).Conclusion Silencing circ0006152 can reduce the malignancy of NSCLC A549 cells by regulating the miR-557/Wnt3a signaling axis.
关键词
非小细胞肺癌/Circ0006152/miR-557/Wnt3a/机制
Key words
Non small cell lung cancer/Circ0006152/miR-557/Wnt3a/Mechanism
万方数据