摘要
目的 采用不同浓度异丙肾上腺素对兔肾脏进行灌注,监测其对兔肾盂压力和生命体征的影响,以探索最佳灌注浓度.方法 将60只新西兰雄性大白兔采用随机抽签方法分成A、B、C、D四组,每组15只,其中A组、B组、C组为实验组,D组为对照组,实验组分别使用浓度为1.0、0.9、0.8 µg/mL的异丙肾上腺素溶液进行肾盂灌注,D组使用生理盐水进行肾盂灌注.记录灌注0、4、8、16、25 min时各组大白兔的肾盂压、平均动脉压、心率及血药浓度.结果 3只大白兔因麻醉过深死亡,在A组、B组及D组各1只,得出57只有效实验数据.4组大白兔肾盂灌注后,肾盂压均升高,实验组与对照组比较,差异有统计学意义(P＜0.05);A、B、C三组灌注至4 min后各组肾盂压力开始下降,D组灌注后肾盂压持续升高,在4、8、16、25 min时4组肾盂压力差异有统计学意义(P＜0.05);在16、25 min时,A、B、C三组间差异有统计学意义(P＜0.05).实验组大白兔肾盂灌注4 min时,平均动脉压、心率、血药浓度数值均高于对照组,差异均有统计学意义(P＜0.05).结论 异丙肾上腺素作为灌注液灌注兔肾脏时可降低肾盂压力,浓度为1.0μg/mL时降低肾盂压力更明显,但影响兔的血压和心率,而在1.0μg/mL以下均为安全浓度.
Abstract
Objective The rabbit kidney was injected with different concentrations of isoproterenol,and the influence of renal pelvis pressure and vital signs was monitored to explore the optimal perfusion concentration.Methods A total of 60 New Zealand male white rabbits were divided into four groups randomly:A,B,C and D,with 15 rabbits in each group.A-mong them,A,B and C were the experimental group and D was the control group,1.0,0.9,0.8 μg/mL isoproterenol so-lution were respectively used for renal pelvis perfusion in the experimental group,while group D received normal saline.Renal pelvis pressure,mean arterial pressure,heart rate and blood drug concentration at the beginning of perfusion,0,4,8,16,25 min following perfusion were recorded.Results Three white rabbits died due to excessive anesthesia,one for each group A,B and D,and the experimental data of 57 white rabbits were obtained.After renal pelvis perfusion,the renal pelvis pressure increased in all four groups,and the difference between the experimental groups and the control group was statistically significant(P＜0.05).The renal pelvis pressure of groups A,B and C began to decrease after 4 minutes of per-fusion,and the renal pelvis pressure of group D continued to increase after perfusion.The difference in renal pelvic pres-sure among the four groups at 4,8,16,25 min was statistically significant(P＜0.05).At 16,25 min,the differences be-tween groups A,B,and C were statistically significant(P＜0.05).After 4 minutes,the mean arterial pressure,heart rate and blood drug concentration of the experimental groups were higher than those of the control group,and the differences were statistically significant(P＜0.05).Conclusion Isoproterenol can reduce the renal pelvis pressure when injected into the rabbit kidney as perfusion solution,more obvious when the concentration was 1.0 μg/mL,but it affects the blood pres-sure and heart rate of rabbits,whereas the concentration below 1.0 µg/mL is safe.
基金项目
安徽省卫生健康委科研项目(AHWJ2021b131)
维普
万方数据