Objective This study investigated and analyzed the influencing factors of pregnancy stress in women with re-fertility,and provided a reference for reducing pregnancy stress in women with re-fertility and improving women's willing-ness to regenerate.Methods From December 2022 to June 2023,320 re-fertile women who underwent obstetric exami-nation in the obstetric clinic of the First Affiliated Hospital of Bengbu Medical University and the Third People's Hospital of Bengbu City were selected as research objects by convenient sampling method.The general information questionnaire,pregnancy stress scale,perceived social support scale and general well-being scale were used to examine the research ob-jects.Results The pregnancy stress score was 0.533(0.325,0.808),the social support score was 62.000(55.000,72.000),and the general well-being score was 79.000(71.000,86.250).There were statistically significant differ-ences in pregnancy stress among women with high-risk pregnancy,marital status,sex of existing offspring,and whether the pregnancy was a high-risk pregnancy(P<0.05).The results of correlation analysis showed that social support(r=-0.338)and general well-being(r=-0.364)were negatively correlated with pregnancy stress(P<0.001).Multiple re-gression analysis showed that marital status,sex of existing offspring,perceived social support and general well-being were important factors influencing pregnancy stress in women with re-fertility(P<0.05).Conclusion Pregnancy stress in women with infertility is generally low.Social support can reduce the pregnancy stress of women with re-fertility and improve the overall well-being of women with re-fertility.Therefore,society,government and medical staff should take relevant measures to reduce the pregnancy stress of re-fertility women,improve women's willingness to reproduce,and promote maternal and child health.